A Genetic Analysis of Intersex, a Gene Regulating Sexual Differentiation in Drosophila Melanogaster Females

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1995 Apr 1

PMID 7789766

Citations 25

Authors

B A Chase

B S Baker

Affiliations

Soon will be listed here.

Abstract

Sex-type in Drosophila melanogaster is controlled by a hierarchically acting set of regulatory genes. At the terminus of this hierarchy lie those regulatory genes responsible for implementing sexual differentiation: genes that control the activity of target loci whose products give rise to sexually dimorphic phenotypes. The genetic analysis of the intersex (ix) gene presented here demonstrates that ix is such a terminally positioned regulatory locus. The ix locus has been localized to the cytogenetic interval between 47E3-6 and 47F11-18. A comparison of the morphological and behavioral phenotypes of homozygotes and hemizygotes for three point mutations at ix indicates that the null phenotype of ix is to transform diplo-X animals into intersexes while leaving haplo-X animals unaffected. Analysis of X-ray induced, mitotic recombination clones lacking ix+ function in the abdomen of diplo-X individuals indicates that the ix+ product functions in a cell-autonomous manner and that it is required at least until the termination of cell division in this tissue. Taken together with previous analyses, our results indicate that the ix+ product is required to function with the female-specific product of doublesex to implement appropriate female sexual differentiation in diplo-X animals.

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References

Cline T . Maternal and zygotic sex-specific gene interactions in Drosophila melanogaster. Genetics. 1980; 96(4):903-26. PMC: 1219308. DOI: 10.1093/genetics/96.4.903. View

Boggs R, Gregor P, Idriss S, Belote J, McKeown M . Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene. Cell. 1987; 50(5):739-47. DOI: 10.1016/0092-8674(87)90332-1. View

Cline T . The interaction between daughterless and sex-lethal in triploids: a lethal sex-transforming maternal effect linking sex determination and dosage compensation in Drosophila melanogaster. Dev Biol. 1983; 95(2):260-74. DOI: 10.1016/0012-1606(83)90027-1. View

Cline T . Autoregulatory functioning of a Drosophila gene product that establish es and maintains the sexually determined state. Genetics. 1984; 107(2):231-77. PMC: 1202321. DOI: 10.1093/genetics/107.2.231. View

Garcia-Bellido A, Dapena J . Induction, detection and characterization of cell differentiation mutants in Drosophila. Mol Gen Genet. 1974; 128(2):117-30. DOI: 10.1007/BF02654485. View

Cline T . A sex-specific, temperature-sensitive maternal effect of the daughterless mutation of Drosophila melanogaster. Genetics. 1976; 84(4):723-42. PMC: 1213604. DOI: 10.1093/genetics/84.4.723. View

Baker B, Ridge K . Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster. Genetics. 1980; 94(2):383-423. PMC: 1214149. DOI: 10.1093/genetics/94.2.383. View

Belote J, Handler A, Wolfner M, Livak K, Baker B . Sex-specific regulation of yolk protein gene expression in Drosophila. Cell. 1985; 40(2):339-48. DOI: 10.1016/0092-8674(85)90148-5. View

McRobert S, Tompkins L . The effect of transformer, doublesex and intersex mutations on the sexual behavior of Drosophila melanogaster. Genetics. 1985; 111(1):89-96. PMC: 1202601. DOI: 10.1093/genetics/111.1.89. View

10.

Shepherd B, Garabedian M, Hung M, Wensink P . Developmental control of Drosophila yolk protein 1 gene by cis-acting DNA elements. Cold Spring Harb Symp Quant Biol. 1985; 50:521-6. DOI: 10.1101/sqb.1985.050.01.064. View

11.

Garabedian M, Shepherd B, Wensink P . A tissue-specific transcription enhancer from the Drosophila yolk protein 1 gene. Cell. 1986; 45(6):859-67. DOI: 10.1016/0092-8674(86)90560-x. View

12.

Cline T . A female-specific lethal lesion in an X-linked positive regulator of the Drosophila sex determination gene, Sex-lethal. Genetics. 1986; 113(3):641-63. PMC: 1202860. DOI: 10.1093/genetics/113.3.641. View

13.

Cronmiller C, Cline T . The Drosophila sex determination gene daughterless has different functions in the germ line versus the soma. Cell. 1987; 48(3):479-87. DOI: 10.1016/0092-8674(87)90198-x. View

14.

Belote J, Baker B . Sexual behavior: its genetic control during development and adulthood in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1987; 84(22):8026-30. PMC: 299469. DOI: 10.1073/pnas.84.22.8026. View

15.

McKeown M, Belote J, Boggs R . Ectopic expression of the female transformer gene product leads to female differentiation of chromosomally male Drosophila. Cell. 1988; 53(6):887-95. DOI: 10.1016/s0092-8674(88)90369-8. View

16.

Cline T . Evidence that sisterless-a and sisterless-b are two of several discrete "numerator elements" of the X/A sex determination signal in Drosophila that switch Sxl between two alternative stable expression states. Genetics. 1988; 119(4):829-62. PMC: 1203469. DOI: 10.1093/genetics/119.4.829. View

17.

Bell L, Maine E, Schedl P, Cline T . Sex-lethal, a Drosophila sex determination switch gene, exhibits sex-specific RNA splicing and sequence similarity to RNA binding proteins. Cell. 1988; 55(6):1037-46. DOI: 10.1016/0092-8674(88)90248-6. View

18.

Oliver B, Perrimon N, Mahowald A . Genetic evidence that the sans fille locus is involved in Drosophila sex determination. Genetics. 1988; 120(1):159-71. PMC: 1203487. DOI: 10.1093/genetics/120.1.159. View

19.

Burtis K, Baker B . Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides. Cell. 1989; 56(6):997-1010. DOI: 10.1016/0092-8674(89)90633-8. View

20.

Baker B . Sex in flies: the splice of life. Nature. 1989; 340(6234):521-4. DOI: 10.1038/340521a0. View