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A Bisulfite Method of 5-methylcytosine Mapping That Minimizes Template Degradation

Overview
Journal Anal Biochem
Publisher Elsevier
Specialty Biochemistry
Date 1995 Mar 20
PMID 7785768
Citations 60
Authors
Affiliations
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Abstract

The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.

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