Transcripts from Opposite Strands of Gamma Satellite DNA Are Differentially Expressed During Mouse Development

Overview

Journal Mamm Genome

Specialty Genetics

Date 1995 Feb 1

PMID 7767009

Citations 55

Authors

F Rudert

S Bronner

J M Garnier

P Dolle

Affiliations

Soon will be listed here.

Abstract

Using in vitro immuno-selected retinoic acid response elements, we have isolated mouse genomic clones containing major (gamma) satellite DNA repeats that are considered as typical of chromosome centromeres. Several cDNA clones were then isolated from a F9 cell cDNA library and were found to harbor variants of the 234-base pair consensus gamma satellite monomer. In Northern analysis, these satellite DNA sequences hybridized predominantly to an approximately 1.8-kb RNA species in polyadenylated RNA from P19 cells. These transcripts were strongly repressed by retinoic acid, and nuclear run-on assays revealed that this repression was, at least in part, mediated at the transcriptional level. Satellite transcripts were also detected in HeLa cells, where they were similarly down-regulated by retinoids. Heterogeneously sized satellite transcripts were detected in RNA from specific mouse tissues, such as fetuses (but not placenta), adult liver, and testis. In situ hybridization analysis revealed that satellite transcripts are generated from opposite DNA strands and are differentially expressed in cells of the developing central nervous system as well as in adult liver and testis. These data may have implications on retinoic acid-mediated transcriptional regulation and centromere function.

Citing Articles

The Proteomic Composition and Organization of Constitutive Heterochromatin in Mouse Tissues.

Schmidt A, Zhang H, Schmitt S, Rausch C, Popp O, Chen J Cells. 2024; 13(2).

PMID: 38247831 PMC: 10814525. DOI: 10.3390/cells13020139.

DOT1L bridges transcription and heterochromatin formation at mammalian pericentromeres.

Malla A, Yu H, Farris D, Kadimi S, Lam T, Cox A EMBO Rep. 2023; 24(8):e56492.

PMID: 37317657 PMC: 10398668. DOI: 10.15252/embr.202256492.

The Evolution of Widespread Recombination Suppression on the Dwarf Hamster (Phodopus) X Chromosome.

Moore E, Thomas G, Mortimer S, Kopania E, Hunnicutt K, Clare-Salzler Z Genome Biol Evol. 2022; 14(6).

PMID: 35642315 PMC: 9185382. DOI: 10.1093/gbe/evac080.

Molecular Dynamics and Evolution of Centromeres in the Genus Equus.

Piras F, Cappelletti E, Santagostino M, Nergadze S, Giulotto E, Raimondi E Int J Mol Sci. 2022; 23(8).

PMID: 35457002 PMC: 9024551. DOI: 10.3390/ijms23084183.

HSF1-Activated Non-Coding Stress Response: Satellite lncRNAs and Beyond, an Emerging Story with a Complex Scenario.

Vourch C, Dufour S, Timcheva K, Seigneurin-Berny D, Verdel A Genes (Basel). 2022; 13(4).

PMID: 35456403 PMC: 9032817. DOI: 10.3390/genes13040597.

References

Draetta G . Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation. Trends Biochem Sci. 1990; 15(10):378-83. DOI: 10.1016/0968-0004(90)90235-4. View

Smith G . Evolution of repeated DNA sequences by unequal crossover. Science. 1976; 191(4227):528-35. DOI: 10.1126/science.1251186. View

Johnson D, Kroisel P, Klapper H, ROSENKRANZ W . Microdissection of a human marker chromosome reveals its origin and a new family of centromeric repetitive DNA. Hum Mol Genet. 1992; 1(9):741-7. DOI: 10.1093/hmg/1.9.741. View

Sleigh M . Differentiation and proliferation in mouse embryonal carcinoma cells. Bioessays. 1992; 14(11):769-75. DOI: 10.1002/bies.950141109. View

Durand B, Saunders M, Leroy P, Leid M, Chambon P . All-trans and 9-cis retinoic acid induction of CRABPII transcription is mediated by RAR-RXR heterodimers bound to DR1 and DR2 repeated motifs. Cell. 1992; 71(1):73-85. DOI: 10.1016/0092-8674(92)90267-g. View

Maraia R . The subset of mouse B1 (Alu-equivalent) sequences expressed as small processed cytoplasmic transcripts. Nucleic Acids Res. 1991; 19(20):5695-702. PMC: 328977. DOI: 10.1093/nar/19.20.5695. View

Zelent A, Mendelsohn C, Kastner P, Krust A, Garnier J, Ruffenach F . Differentially expressed isoforms of the mouse retinoic acid receptor beta generated by usage of two promoters and alternative splicing. EMBO J. 1991; 10(1):71-81. PMC: 452613. DOI: 10.1002/j.1460-2075.1991.tb07922.x. View

Schrewe H, Thompson J, Bona M, Hefta L, Maruya A, Hassauer M . Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression. Mol Cell Biol. 1990; 10(6):2738-48. PMC: 360634. DOI: 10.1128/mcb.10.6.2738-2748.1990. View

Linial M, Gunderson N, Groudine M . Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis. Science. 1985; 230(4730):1126-32. DOI: 10.1126/science.2999973. View

10.

Jackson M, Mole S, Ponder B . Characterisation of a boundary between satellite III and alphoid sequences on human chromosome 10. Nucleic Acids Res. 1992; 20(18):4781-7. PMC: 334232. DOI: 10.1093/nar/20.18.4781. View

11.

Vissel B, Choo K . Mouse major (gamma) satellite DNA is highly conserved and organized into extremely long tandem arrays: implications for recombination between nonhomologous chromosomes. Genomics. 1989; 5(3):407-14. DOI: 10.1016/0888-7543(89)90003-7. View

12.

Butner K, Lo C . High frequency DNA rearrangements associated with mouse centromeric satellite DNA. J Mol Biol. 1986; 187(4):547-56. DOI: 10.1016/0022-2836(86)90333-5. View

13.

Haigwood N, Jahn C, HUTCHISON 3rd C, Edgell M . Locations of three repetitive sequence families found in BALB/c adult beta-globin clones. Nucleic Acids Res. 1981; 9(5):1133-50. PMC: 326742. DOI: 10.1093/nar/9.5.1133. View

14.

Auffray C, Rougeon F . Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980; 107(2):303-14. DOI: 10.1111/j.1432-1033.1980.tb06030.x. View

15.

de The H, Tiollais P, Stunnenberg H, Dejean A . Identification of a retinoic acid responsive element in the retinoic acid receptor beta gene. Nature. 1990; 343(6254):177-80. DOI: 10.1038/343177a0. View

16.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

17.

Rudert F, Garnier J, Schuhbaur B . Cloning a pseudogene and cDNA encoding a 17-kDa ribosomal protein from mouse: structure and regulation of expression. Gene. 1993; 133(2):249-54. DOI: 10.1016/0378-1119(93)90647-l. View

18.

De Luca L . Retinoids and their receptors in differentiation, embryogenesis, and neoplasia. FASEB J. 1991; 5(14):2924-33. View

19.

Ragsdale Jr C, Brockes J . Retinoids and their targets in vertebrate development. Curr Opin Cell Biol. 1991; 3(6):928-34. DOI: 10.1016/0955-0674(91)90109-c. View

20.

Stephenson E, Erba H, Gall J . Histone gene clusters of the newt notophthalmus are separated by long tracts of satellite DNA. Cell. 1981; 24(3):639-47. DOI: 10.1016/0092-8674(81)90090-8. View