Production of Monoclonal Antibodies and Enzyme Immunoassay for Typical Adenylate Cyclase Activator, Forskolin

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 1994 Jan 1

PMID 7765788

Citations 15

Authors

R Sakata

Y Shoyama

H Murakami

Affiliations

Soon will be listed here.

Abstract

The ratio of hapten to bovine serum albumin in an antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against forskolin was produced by fusing splenocytes immunized with a forskolin-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cells. The cross-reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.57%. A very small cross-reaction appeared with 1-deoxy, 9-deoxy and 1,9-dideoxy forskolin derivatives. The full measuring range of the assay extends from 6 ng to 200 ng of forskolin. The competitive ELISA assay used for this analysis was found to be more sensitive than TLC (10 micrograms), GLC (30 ng) and HPLC (1 microgram) methods.

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