Cloning and Expression of a Pathway for Benzene and Toluene from Bacillus Stearothermophilus

Overview

Journal Biodegradation

Specialties Biochemistry
Environmental Health

Date 1994 Jun 1

PMID 7765116

Citations 3

Authors

M R NATARAJAN

Z Lu

P Oriel

Affiliations

Soon will be listed here.

Abstract

Bacillus stearothermophilus strain BR325 demonstrating broad aromatic substrate capability was isolated from petroleum-contaminated soil. The chromosomally-located aromatic pathway from this isolate was cloned into Escherichia coli as a 32 kb insert in cosmid pHC79, conferring growth on benzene, phenol, and toluene as sole carbon sources.

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References

Gurujeyalakshmi G, Oriel P . Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase. Appl Environ Microbiol. 1989; 55(2):500-2. PMC: 184139. DOI: 10.1128/aem.55.2.500-502.1989. View

Zylstra G, Gibson D . Aromatic hydrocarbon degradation: a molecular approach. Genet Eng (N Y). 1991; 13:183-203. DOI: 10.1007/978-1-4615-3760-1_8. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Saito H, MIURA K . PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963; 72:619-29. View

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

Kojima Y, ITADA N, HAYAISHI O . Metapyrocatachase: a new catechol-cleaving enzyme. J Biol Chem. 1961; 236:2223-8. View

Keil H, Keil S, Pickup R, Williams P . Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53. J Bacteriol. 1985; 164(2):887-95. PMC: 214335. DOI: 10.1128/jb.164.2.887-895.1985. View

Kukor J, Olsen R . Genetic organization and regulation of a meta cleavage pathway for catechols produced from catabolism of toluene, benzene, phenol, and cresols by Pseudomonas pickettii PKO1. J Bacteriol. 1991; 173(15):4587-94. PMC: 208133. DOI: 10.1128/jb.173.15.4587-4594.1991. View

Dong F, Wang L, Wang C, Cheng J, He Z, Sheng Z . Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli. Appl Environ Microbiol. 1992; 58(8):2531-5. PMC: 195817. DOI: 10.1128/aem.58.8.2531-2535.1992. View

10.

NATARAJAN M, Oriel P . Conjugal transfer of recombinant transposon Tn916 from Escherichia coli to Bacillus stearothermophilus. Plasmid. 1991; 26(1):67-73. DOI: 10.1016/0147-619x(91)90037-w. View

11.

Buswell J . Metabolism of phenol and cresols by Bacillus stearothermophilus. J Bacteriol. 1975; 124(3):1077-83. PMC: 236008. DOI: 10.1128/jb.124.3.1077-1083.1975. View

12.

Strom P . Identification of thermophilic bacteria in solid-waste composting. Appl Environ Microbiol. 1985; 50(4):906-13. PMC: 291767. DOI: 10.1128/aem.50.4.906-913.1985. View