Purification and Properties of Collagenase from Cytophaga Sp. L43-1 Strain

Overview

Journal Biosci Biotechnol Biochem

Publisher Oxford University Press

Specialties Biochemistry
Biotechnology

Date 1993 Nov 1

PMID 7764338

Citations 4

Authors

Y Sasagawa

Y Kamio

Y Matsubara

K Suzuki

H Kojima

K Izaki

Affiliations

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Abstract

A collagenolytic bacterial strain was isolated from soil and was identified as Cytophaga sp. It produced several kinds of collagenase and protease. From the supernatant of a culture, a collagenase was purified as a single protein band upon SDS-PAGE and its molecular mass was estimated to be 120 kDa. Collagen and gelatin were good substrates for this enzyme. beta-Casein was cleaved by this enzyme at several sites.

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