Stability and Activity of a Phenol Oxidase from the Ligninolytic Fungus Pleurotus Ostreatus

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 1993 Jul 1

PMID 7763931

Citations 29

Authors

G Palmieri

P Giardina

L Marzullo

B Desiderio

G NITTI

R Cannio

G Sannia

Affiliations

Soon will be listed here.

Abstract

Three different phenol oxidases produced by the basidiomycete fungus Pleurotus ostreatus have been isolated and their main structural, enzymatic and physico-chemical properties characterized. Studies have focused on the most abundantly secreted of these proteins, a copper-enzyme specific towards ortho-diphenol substrates. This protein was purified to homogeneity and part of its primary structure determined by direct protein sequencing. The influence of pH, temperature and presence of water-soluble or water-insoluble organic solvents on the activity and stability of the enzyme were also investigated. These data can be used for applying bioreactors to problems of environmental concern such as waste-water treatment.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Bourbonnais R, Paice M . Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation. FEBS Lett. 1990; 267(1):99-102. DOI: 10.1016/0014-5793(90)80298-w. View

Oakley B, Kirsch D, Morris N . A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980; 105(2):361-3. DOI: 10.1016/0003-2697(80)90470-4. View

Tien M, Kirk T . Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. Proc Natl Acad Sci U S A. 1984; 81(8):2280-4. PMC: 345042. DOI: 10.1073/pnas.81.8.2280. View

Hunkapiller M, HOOD L . Protein sequence analysis: automated microsequencing. Science. 1983; 219(4585):650-9. DOI: 10.1126/science.6687410. View

Kawai S, Umezawa T, Shimada M, Higuchi T . Aromatic ring cleavage of 4,6-di(tert-butyl)guaiacol, a phenolic lignin model compound, by laccase of Coriolus versicolor. FEBS Lett. 1988; 236(2):309-11. DOI: 10.1016/0014-5793(88)80043-7. View

Sannia G, Limongi P, Cocca E, Buonocore F, NITTI G, Giardina P . Purification and characterization of a veratryl alcohol oxidase enzyme from the lignin degrading basidiomycete Pleurotus ostreatus. Biochim Biophys Acta. 1991; 1073(1):114-9. DOI: 10.1016/0304-4165(91)90190-r. View

Badiani M, Felici M, Luna M, Artemi F . Laccase assay by means of high-performance liquid chromatography. Anal Biochem. 1983; 133(2):275-6. DOI: 10.1016/0003-2697(83)90082-9. View

Saloheimo M, Niku-Paavola M, Knowles J . Isolation and structural analysis of the laccase gene from the lignin-degrading fungus Phlebia radiata. J Gen Microbiol. 1991; 137(7):1537-44. DOI: 10.1099/00221287-137-7-1537. View

10.

Kawai S, Umezawa T, Higuchi T . Degradation mechanisms of phenolic beta-1 lignin substructure model compounds by laccase of Coriolus versicolor. Arch Biochem Biophys. 1988; 262(1):99-110. DOI: 10.1016/0003-9861(88)90172-5. View

11.

Dorn E, Hellwig M, Reineke W, Knackmuss H . Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974; 99(1):61-70. DOI: 10.1007/BF00696222. View

12.

Valli K, Gold M . Degradation of 2,4-dichlorophenol by the lignin-degrading fungus Phanerochaete chrysosporium. J Bacteriol. 1991; 173(1):345-52. PMC: 207193. DOI: 10.1128/jb.173.1.345-352.1991. View

13.

Kojima Y, Tsukuda Y, Kawai Y, Tsukamoto A, Sugiura J, Sakaino M . Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. J Biol Chem. 1990; 265(25):15224-30. View