Characterization of Five Esterases from Listeria Monocytogenes and Use of Their Electrophoretic Polymorphism for Strain Typing

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1995 Apr 1

PMID 7747982

Citations 3

Authors

P Gilot

P Andre

Affiliations

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Abstract

Esterases from Listeria monocytogenes strains isolated from cheeses were analyzed by starch gel electrophoresis. Five esterases, numbered from EST 1 to EST 5 in order of decreasing anodal migration, were identified. The EST 1, EST 3, EST 4, and EST 5 set was most active toward alpha-naphthyl propionate, while EST 2 was most active toward alpha-naphthyl acetate. Results from inhibitor studies suggest that all of these esterases were EC class 3.1.1.1 carboxylesterases, except that EST 1 and EST 3 also showed some sensitivity to parahydroxymercuribenzoate. Polymorphism of these five esterases was observed in the population. Twelve esterase patterns were defined and used to subdivide serotypes.

Citing Articles

Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.

Jersek B, Gilot P, Gubina M, Klun N, Mehle J, Tcherneva E J Clin Microbiol. 1998; 37(1):103-9.

PMID: 9854072 PMC: 84179. DOI: 10.1128/JCM.37.1.103-109.1999.

Characterization of Listeria monocytogenes isolates by esterase electrophoresis.

Harvey J, Gilmour A Appl Environ Microbiol. 1996; 62(4):1461-6.

PMID: 8919813 PMC: 167918. DOI: 10.1128/aem.62.4.1461-1466.1996.

Serotyping and esterase typing for analysis of Listeria monocytogenes populations recovered from foodstuffs and from human patients with listeriosis in Belgium.

Gilot P, Genicot A, Andre P J Clin Microbiol. 1996; 34(4):1007-10.

PMID: 8815071 PMC: 228941. DOI: 10.1128/jcm.34.4.1007-1010.1996.

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