Cloning and Characterization of the Bacillus Subtilis BirA Gene Encoding a Repressor of the Biotin Operon

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 May 1

PMID 7730294

Citations 25

Authors

S Bower

J Perkins

R R Yocum

P Serror

A Sorokin

P Rahaim

C L Howitt

N Prasad

S D Ehrlich

J Pero

Affiliations

Soon will be listed here.

Abstract

The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.

Citing Articles

Biotin protein ligase as you like it: Either extraordinarily specific or promiscuous protein biotinylation.

Cronan J Proteins. 2023; 92(4):435-448.

PMID: 37997490 PMC: 10932917. DOI: 10.1002/prot.26642.

The Catabolic System of Acetovanillone and Acetosyringone in sp. Strain SYK-6 Useful for Upgrading Aromatic Compounds Obtained through Chemical Lignin Depolymerization.

Higuchi Y, Kamimura N, Takenami H, Kikuiri Y, Yasuta C, Tanatani K Appl Environ Microbiol. 2022; 88(16):e0072422.

PMID: 35938864 PMC: 9397112. DOI: 10.1128/aem.00724-22.

Genomic Data of Nodule Ensifer mexicanus ITTG R7.

Rincon-Rosales R, Rogel M, Guerrero G, Rincon-Molina C, Lopez-Lopez A, Manzano-Gomez L Microbiol Resour Announc. 2021; 10(13).

PMID: 33795348 PMC: 8104056. DOI: 10.1128/MRA.01251-20.

Specificity and selectivity in post-translational biotin addition.

Beckett D Biochem Soc Trans. 2018; 46(6):1577-1591.

PMID: 30381340 PMC: 7703869. DOI: 10.1042/BST20180425.

Critical Minireview: The Fate of tRNA during Oxidative Stress in Bacillus subtilis.

Guillen J, Jones G, Saldana Gutierrez C, Hernandez-Flores J, Cruz Medina J, Valenzuela Soto J Biomolecules. 2017; 7(1).

PMID: 28117687 PMC: 5372718. DOI: 10.3390/biom7010006.

References

Dubnau D . Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J Mol Biol. 1971; 56(2):209-21. DOI: 10.1016/0022-2836(71)90460-8. View

Suzuki Y, Aoki Y, Ishida Y, Chiba Y, Iwamatsu A, Kishino T . Isolation and characterization of mutations in the human holocarboxylase synthetase cDNA. Nat Genet. 1994; 8(2):122-8. DOI: 10.1038/ng1094-122. View

PAI C . Biochemical and genetic characterization of dehydrobiotin resistant mutants of Escherichia coli. Mol Gen Genet. 1974; 134(4):345-57. DOI: 10.1007/BF00337469. View

PAI C . Genetics of biotin biosynthesis in Bacillus subtilis. J Bacteriol. 1975; 121(1):1-8. PMC: 285605. DOI: 10.1128/jb.121.1.1-8.1975. View

Eisenburg M, Mee B, Prakash O, Eisenburg M . Properties of alpha-dehydrobiotin-resistant mutants of Escherichia coli K-12. J Bacteriol. 1975; 122(1):66-72. PMC: 235640. DOI: 10.1128/jb.122.1.66-72.1975. View

Izumi Y, Ogata K . Some aspects of the microbial production of biotin. Adv Appl Microbiol. 1977; 22:145-76. DOI: 10.1016/s0065-2164(08)70162-x. View

Barker D, Campbell A . Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli. J Bacteriol. 1980; 143(2):789-800. PMC: 294364. DOI: 10.1128/jb.143.2.789-800.1980. View

Barker D, Campbell A . Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981; 146(4):469-92. DOI: 10.1016/0022-2836(81)90043-7. View

EISENBERG M, Prakash O, Hsiung S . Purification and properties of the biotin repressor. A bifunctional protein. J Biol Chem. 1982; 257(24):15167-73. View

10.

Youngman P, Perkins J, Losick R . Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid. 1984; 12(1):1-9. DOI: 10.1016/0147-619x(84)90061-1. View

11.

EISENBERG M . Regulation of the biotin operon in E. coli. Ann N Y Acad Sci. 1985; 447:335-49. DOI: 10.1111/j.1749-6632.1985.tb18449.x. View

12.

Howard P, Shaw J, Otsuka A . Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli. Gene. 1985; 35(3):321-31. DOI: 10.1016/0378-1119(85)90011-3. View

13.

Buoncristiani M, Howard P, Otsuka A . DNA-binding and enzymatic domains of the bifunctional biotin operon repressor (BirA) of Escherichia coli. Gene. 1986; 44(2-3):255-61. DOI: 10.1016/0378-1119(86)90189-7. View

14.

Shiuan D, Campbell A . Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons. Gene. 1988; 67(2):203-11. DOI: 10.1016/0378-1119(88)90397-6. View

15.

Cronan Jr J . The E. coli bio operon: transcriptional repression by an essential protein modification enzyme. Cell. 1989; 58(3):427-9. DOI: 10.1016/0092-8674(89)90421-2. View

16.

Gloeckler R, Ohsawa I, Speck D, Ledoux C, Bernard S, Zinsius M . Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin. Gene. 1990; 87(1):63-70. DOI: 10.1016/0378-1119(90)90496-e. View

17.

Lauer G, Rudd E, McKay D, Ally A, Ally D, Backman K . Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase. J Bacteriol. 1991; 173(16):5047-53. PMC: 208194. DOI: 10.1128/jb.173.16.5047-5053.1991. View

18.

Speck D, Ohsawa I, Gloeckler R, Zinsius M, Bernard S, Ledoux C . Isolation of Bacillus sphaericus biotin synthesis control mutants: evidence for transcriptional regulation of bio genes. Gene. 1991; 108(1):39-45. DOI: 10.1016/0378-1119(91)90485-t. View

19.

Wilson K, Shewchuk L, Brennan R, Otsuka A, Matthews B . Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains. Proc Natl Acad Sci U S A. 1992; 89(19):9257-61. PMC: 50105. DOI: 10.1073/pnas.89.19.9257. View

20.

Xu X, Yagi T . DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans. Biochemistry. 1993; 32(3):968-81. DOI: 10.1021/bi00054a030. View