Cloning, Sequencing, and Characterization of the Gene Encoding FrpB, a Major Iron-regulated, Outer Membrane Protein of Neisseria Gonorrhoeae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Apr 1

PMID 7721696

Citations 36

Authors

M Beucher

P F Sparling

Affiliations

Soon will be listed here.

Abstract

FrpB (for Fe-regulated protein B) is a 76-kDa outer membrane protein that is part of the iron regulon of Neisseria gonorrhoeae and Neisseria meningitidis. The frpB gene from gonococcal strain FA19 was cloned and sequenced. FrpB was homologous to several TonB-dependent outer membrane receptors of Escherichia coli as well as HemR of Yersinia enterocolitica and CopB of Moraxella catarrhalis. An omga insertion into the frpB coding sequence caused a 60% reduction in 55Fe uptake from heme, but careful analysis suggested that this effect was nonspecific. While FrpB was related to the family of TonB-dependent proteins, a function in iron uptake could not be documented.

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References

Klebba P, McIntosh M, NEILANDS J . Kinetics of biosynthesis of iron-regulated membrane proteins in Escherichia coli. J Bacteriol. 1982; 149(3):880-8. PMC: 216474. DOI: 10.1128/jb.149.3.880-888.1982. View

Elkins C, Carbonetti N, Varela V, Stirewalt D, Klapper D, Sparling P . Antibodies to N-terminal peptides of gonococcal porin are bactericidal when gonococcal lipopolysaccharide is not sialylated. Mol Microbiol. 1992; 6(18):2617-28. DOI: 10.1111/j.1365-2958.1992.tb01439.x. View

Weinberg E . Iron withholding: a defense against infection and neoplasia. Physiol Rev. 1984; 64(1):65-102. DOI: 10.1152/physrev.1984.64.1.65. View

Stern A, Nickel P, Meyer T, So M . Opacity determinants of Neisseria gonorrhoeae: gene expression and chromosomal linkage to the gonococcal pilus gene. Cell. 1984; 37(2):447-56. DOI: 10.1016/0092-8674(84)90375-1. View

Prentki P, Krisch H . In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984; 29(3):303-13. DOI: 10.1016/0378-1119(84)90059-3. View

West S, Sparling P . Response of Neisseria gonorrhoeae to iron limitation: alterations in expression of membrane proteins without apparent siderophore production. Infect Immun. 1985; 47(2):388-94. PMC: 263181. DOI: 10.1128/iai.47.2.388-394.1985. View

Streisinger G, Owen J . Mechanisms of spontaneous and induced frameshift mutation in bacteriophage T4. Genetics. 1985; 109(4):633-59. PMC: 1202499. DOI: 10.1093/genetics/109.4.633. View

Seifert H, Chen E, So M, Heffron F . Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986; 83(3):735-9. PMC: 322939. DOI: 10.1073/pnas.83.3.735. View

Biswas G, Graves J, Schwalbe R, Sparling P . Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid. J Bacteriol. 1986; 167(2):685-94. PMC: 212944. DOI: 10.1128/jb.167.2.685-694.1986. View

10.

Lundrigan M, Kadner R . Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB. J Biol Chem. 1986; 261(23):10797-801. View

11.

Black J, Dyer D, Thompson M, Sparling P . Human immune response to iron-repressible outer membrane proteins of Neisseria meningitidis. Infect Immun. 1986; 54(3):710-3. PMC: 260227. DOI: 10.1128/iai.54.3.710-713.1986. View

12.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

13.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

14.

Sonntag I, Schwarz H, Hirota Y, Henning U . Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins. J Bacteriol. 1978; 136(1):280-5. PMC: 218658. DOI: 10.1128/jb.136.1.280-285.1978. View

15.

Weinberg E . Iron and infection. Microbiol Rev. 1978; 42(1):45-66. PMC: 281418. DOI: 10.1128/mr.42.1.45-66.1978. View

16.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

17.

Archibald F, DeVoe I . Iron acquisition by Neisseria meningitidis in vitro. Infect Immun. 1980; 27(2):322-34. PMC: 550768. DOI: 10.1128/iai.27.2.322-334.1980. View

18.

NEILANDS J . Microbial iron compounds. Annu Rev Biochem. 1981; 50:715-31. DOI: 10.1146/annurev.bi.50.070181.003435. View

19.

Warner P, Williams P, Bindereif A, NEILANDS J . ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981; 33(2):540-5. PMC: 350734. DOI: 10.1128/iai.33.2.540-545.1981. View

20.

Mickelsen P, Sparling P . Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infect Immun. 1981; 33(2):555-64. PMC: 350736. DOI: 10.1128/iai.33.2.555-564.1981. View