Detection and Subsequent Sequencing of Puumala Virus from Human Specimens by PCR

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1995 Feb 1

PMID 7714178

Citations 11

Authors

J Horling

A Lundkvist

K Persson

M Mullaart

T Dzagurova

A Dekonenko

E Tkachenko

B Niklasson

Affiliations

Soon will be listed here.

Abstract

A sensitive method based on PCR was developed for the detection of Puumala virus (PUU) in human samples. The assay was found to be specific for PUU-like strains and distinguished between these and hantaviruses of other serotypes. The detection limit was found to be 10(-5) focus-forming units. Clinical samples were collected from patients with nephropathia epidemica in Sweden and western Russia. Five whole blood samples collected from patients in Russia with the acute phase of disease were found to be positive by the PCR. All samples were negative for PUU antigen when examined by enzyme-linked immunosorbent assay. Virus isolation on Vero E6 cells from several of the acute-phase samples, including the 5 PCR-positive samples, was not successful. The amplified samples were subjected to direct nucleic acid sequencing for confirmation of identity. The sequences differed from each other and were closely related to the Russian bank vole isolate CG-1820, thereby indicating the origin of nephropathia epidemica. The PCR was used for amplification and subsequent nucleotide sequencing of eight PUU-like isolates with different geographic origins. The Swedish strains were more closely related to the Finnish PUU prototype strain, Sotkamo, than to the Russian isolates. Interestingly, a Belgian isolate, CG-13891, differed markedly from all other PUU strains.

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References

Stohwasser R, Giebel L, Zoller L, Bautz E, Darai G . Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hällnäs B1. Virology. 1990; 174(1):79-86. DOI: 10.1016/0042-6822(90)90056-w. View

Gligic A, Frusic M, Obradovic M, Stojanovic R, Hlaca D, Gibbs Jr C . Hemorrhagic fever with renal syndrome in Yugoslavia: antigenic characterization of hantaviruses isolated from Apodemus flavicollis and Clethrionomys glareolus. Am J Trop Med Hyg. 1989; 41(1):109-15. View

Elliott R . Molecular biology of the Bunyaviridae. J Gen Virol. 1990; 71 ( Pt 3):501-22. DOI: 10.1099/0022-1317-71-3-501. View

Gu X, You Z, Meng G, Luo F, Yang S, Yang S . Hemorrhagic fever with renal syndrome. Separation of human peripheral blood T and B cells and detection of viral antigen. Chin Med J (Engl). 1990; 103(1):25-8. View

Giebel L, Zoller L, Bautz E, Darai G . Rapid detection of genomic variations in different strains of hantaviruses by polymerase chain reaction techniques and nucleotide sequence analysis. Virus Res. 1990; 16(2):127-36. DOI: 10.1016/0168-1702(90)90017-6. View

Niklasson B, Tkachenko E, Ivanov A, van der Groen G, Wiger D, Andersen H . Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM. Res Virol. 1990; 141(6):637-48. DOI: 10.1016/0923-2516(90)90036-i. View

Xiao S, Yanagihara R, Godec M, Eldadah Z, Johnson B, Gajdusek D . Detection of hantavirus RNA in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction. J Med Virol. 1991; 33(4):277-82. DOI: 10.1002/jmv.1890330413. View

Antic D, Lim B, Kang C . Nucleotide sequence and coding capacity of the large (L) genomic RNA segment of Seoul 80-39 virus, a member of the hantavirus genus. Virus Res. 1991; 19(1):59-65. DOI: 10.1016/0168-1702(91)90094-c. View

Holodniy M, Kim S, Katzenstein D, Konrad M, Groves E, Merigan T . Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol. 1991; 29(4):676-9. PMC: 269852. DOI: 10.1128/jcm.29.4.676-679.1991. View

10.

Lundkvist A, Fatouros A, Niklasson B . Antigenic variation of European haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies. J Gen Virol. 1991; 72 ( Pt 9):2097-103. DOI: 10.1099/0022-1317-72-9-2097. View

11.

Niklasson B, Jonsson M, Lundkvist A, Horling J, Tkachenko E . Comparison of European isolates of viruses causing hemorrhagic fever with renal syndrome by a neutralization test. Am J Trop Med Hyg. 1991; 45(6):660-5. DOI: 10.4269/ajtmh.1991.45.660. View

12.

Grankvist O, Juto P, Settergren B, Ahlm C, Bjermer L, Linderholm M . Detection of nephropathia epidemica virus RNA in patient samples using a nested primer-based polymerase chain reaction. J Infect Dis. 1992; 165(5):934-7. DOI: 10.1093/infdis/165.5.934. View

13.

Vapalahti O, Kallio-Kokko H, Salonen E, BRUMMER-KORVENKONTIO M, Vaheri A . Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein. J Gen Virol. 1992; 73 ( Pt 4):829-38. DOI: 10.1099/0022-1317-73-4-829. View

14.

Arthur R, Lofts R, Gomez J, Glass G, LeDuc J, Childs J . Grouping of hantaviruses by small (S) genome segment polymerase chain reaction and amplification of viral RNA from wild-caught rats. Am J Trop Med Hyg. 1992; 47(2):210-24. DOI: 10.4269/ajtmh.1992.47.210. View

15.

Horling J, Lundkvist A, Huggins J, Niklasson B . Antibodies to Puumala virus in humans determined by neutralization test. J Virol Methods. 1992; 39(1-2):139-47. DOI: 10.1016/0166-0934(92)90132-w. View

16.

Puthavathana P, Lee H, Kang C . Typing of Hantaviruses from five continents by polymerase chain reaction. Virus Res. 1992; 26(1):1-14. DOI: 10.1016/0168-1702(92)90142-v. View

17.

Avsic-Zupanc T, Xiao S, Stojanovic R, Gligic A, van der Groen G, LeDuc J . Characterization of Dobrava virus: a Hantavirus from Slovenia, Yugoslavia. J Med Virol. 1992; 38(2):132-7. DOI: 10.1002/jmv.1890380211. View

18.

Lundkvist A, Bjorsten S, Niklasson B . Immunoglobulin G subclass responses against the structural components of Puumala virus. J Clin Microbiol. 1993; 31(2):368-72. PMC: 262767. DOI: 10.1128/jcm.31.2.368-372.1993. View

19.

Lundkvist A, Horling J, Niklasson B . The humoral response to Puumala virus infection (nephropathia epidemica) investigated by viral protein specific immunoassays. Arch Virol. 1993; 130(1-2):121-30. DOI: 10.1007/BF01319001. View

20.

Horling J, Vene S, Franzen C, Niklasson B . Detection of Ockelbo virus RNA in skin biopsies by polymerase chain reaction. J Clin Microbiol. 1993; 31(8):2004-9. PMC: 265687. DOI: 10.1128/jcm.31.8.2004-2009.1993. View