Formalin Fixation and Patterns of Antineutrophil Cytoplasmic Antibodies

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1995 Jan 1

PMID 7706530

Citations 10

Authors

G P Spickett

V Broomhead

Affiliations

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Abstract

Recent publications have suggested that fixing neutrophils in formalin is a useful adjunct to the differentiation of antinuclear antibodies from perinuclear antineutrophil cytoplasmic antibodies. In this small comparative study of 30 sera the use of an additional slide of formalin fixed neutrophils did not add any useful information and may be confusing as the results were not reproducible.

Citing Articles

Role of auto-antibodies for the diagnosis of chronic cholestatic liver diseases.

Terjung B, Spengler U Clin Rev Allergy Immunol. 2005; 28(2):115-33.

PMID: 15879618 DOI: 10.1385/CRIAI:28:2:115.

Differentiation of antineutrophil nuclear antibodies in inflammatory bowel and autoimmune liver diseases from antineutrophil cytoplasmic antibodies (p-ANCA) using immunofluorescence microscopy.

Terjung B, Worman H, Herzog V, Sauerbruch T, Spengler U Clin Exp Immunol. 2001; 126(1):37-46.

PMID: 11678897 PMC: 1906166. DOI: 10.1046/j.1365-2249.2001.01649.x.

Pitfalls of formalin fixation for determination of antineutrophil cytoplasmic antibodies.

Chowdhury S, Broomhead V, Spickett G, Wilkinson R J Clin Pathol. 1999; 52(6):475-7.

PMID: 10562820 PMC: 501439. DOI: 10.1136/jcp.52.6.475.

Immunofluorescent patterns associated with ANCA.

Lock R, Unsworth D J Clin Pathol. 1999; 52(3):237.

PMID: 10450189 PMC: 501096. DOI: 10.1136/jcp.52.3.237b.

How to test for antineutrophil cytoplasmic antibodies--evidence based immunology?.

Spickett G, Broomhead V J Clin Pathol. 1999; 52(2):81-2.

PMID: 10396231 PMC: 501043. DOI: 10.1136/jcp.52.2.81.

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