Allelic Variation in the Helicobacter Pylori Flagellin Genes FlaA and FlaB: Its Consequences for Strain Typing Schemes and Population Structure

Overview

Journal Epidemiol Infect

Publisher Cambridge University Press

Specialties Infectious Diseases
Public Health

Date 1995 Apr 1

PMID 7705489

Citations 13

Authors

K J Forbes

Z Fang

T H Pennington

Affiliations

Soon will be listed here.

Abstract

Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI, HindIII, MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.

Citing Articles

Antigenic and conserved peptides from diverse Helicobacter pylori antigens.

Calado C Biotechnol Lett. 2022; 44(3):535-545.

PMID: 35277779 PMC: 8916697. DOI: 10.1007/s10529-022-03238-x.

PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients.

Menoni S, Bonon S, Zeitune J, Costa S Gastroenterol Res Pract. 2013; 2013:951034.

PMID: 23401678 PMC: 3562691. DOI: 10.1155/2013/951034.

Polymorphism of flagellin A gene in Helicobacter pylori.

Ji W, Hu J, Qiu J, Peng D, Shi B, Zhou S World J Gastroenterol. 2002; 7(6):783-7.

PMID: 11854901 PMC: 4695594. DOI: 10.3748/wjg.v7.i6.783.

Comparison of genetic divergence and fitness between two subclones of Helicobacter pylori.

Bjorkholm B, Lundin A, Sillen A, Guillemin K, Salama N, Rubio C Infect Immun. 2001; 69(12):7832-8.

PMID: 11705965 PMC: 98879. DOI: 10.1128/IAI.69.12.7832-7838.2001.

Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: experimental results and meta-analysis.

Burucoa C, Lhomme V, Fauchere J J Clin Microbiol. 1999; 37(12):4071-80.

PMID: 10565934 PMC: 85883. DOI: 10.1128/JCM.37.12.4071-4080.1999.

References

ORourke M, Stevens E . Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J Gen Microbiol. 1993; 139(11):2603-11. DOI: 10.1099/00221287-139-11-2603. View

Wang Y, Roos K, Taylor D . Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. J Gen Microbiol. 1993; 139(10):2485-93. DOI: 10.1099/00221287-139-10-2485. View

ORourke M, Spratt B . Further evidence for the non-clonal population structure of Neisseria gonorrhoeae: extensive genetic diversity within isolates of the same electrophoretic type. Microbiology (Reading). 1994; 140 ( Pt 6):1285-90. DOI: 10.1099/00221287-140-6-1285. View

Marshall B, Warren J . Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984; 1(8390):1311-5. DOI: 10.1016/s0140-6736(84)91816-6. View

Langenberg W, Rauws E, Widjojokusumo A, Tytgat G, Zanen H . Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol. 1986; 24(3):414-7. PMC: 268925. DOI: 10.1128/jcm.24.3.414-417.1986. View

Majewski S, Goodwin C . Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation. J Infect Dis. 1988; 157(3):465-71. DOI: 10.1093/infdis/157.3.465. View

Eaton K, Morgan D, Krakowka S . Campylobacter pylori virulence factors in gnotobiotic piglets. Infect Immun. 1989; 57(4):1119-25. PMC: 313239. DOI: 10.1128/iai.57.4.1119-1125.1989. View

Bukholm G, BOVRE K . Natural competence for genetic transformation in Campylobacter pylori. J Infect Dis. 1990; 161(2):365-6. DOI: 10.1093/infdis/161.2.365. View

Musser J, Kroll J, Granoff D, Moxon E, Brodeur B, Campos J . Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis. 1990; 12(1):75-111. DOI: 10.1093/clinids/12.1.75. View

10.

Owen R, Fraser J, Costas M, Morgan D, Morgan D . Signature patterns of DNA restriction fragments of Helicobacter pylori before and after treatment. J Clin Pathol. 1990; 43(8):646-9. PMC: 502646. DOI: 10.1136/jcp.43.8.646. View

11.

Forman D, Newell D, Fullerton F, Yarnell J, Stacey A, Wald N . Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. BMJ. 1991; 302(6788):1302-5. PMC: 1670011. DOI: 10.1136/bmj.302.6788.1302. View

12.

Taylor D, Eaton M, Chang N, Salama S . Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J Bacteriol. 1992; 174(21):6800-6. PMC: 207355. DOI: 10.1128/jb.174.21.6800-6806.1992. View

13.

Forbes K, Bruce K, Ball A, Pennington T . Variation in length and sequence of porin (ompP2) alleles of non-capsulate Haemophilus influenzae. Mol Microbiol. 1992; 6(15):2107-12. DOI: 10.1111/j.1365-2958.1992.tb01384.x. View

14.

Akopyanz N, Bukanov N, Westblom T, Kresovich S, Berg D . DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res. 1992; 20(19):5137-42. PMC: 334296. DOI: 10.1093/nar/20.19.5137. View

15.

Leying H, Suerbaum S, Geis G, Haas R . Cloning and genetic characterization of a Helicobacter pylori flagellin gene. Mol Microbiol. 1992; 6(19):2863-74. DOI: 10.1111/j.1365-2958.1992.tb01466.x. View

16.

Nwokolo C, Bickley J, Attard A, Owen R, Costas M, Fraser I . Evidence of clonal variants of Helicobacter pylori in three generations of a duodenal ulcer disease family. Gut. 1992; 33(10):1323-7. PMC: 1379597. DOI: 10.1136/gut.33.10.1323. View

17.

Akopyanz N, Bukanov N, Westblom T, Berg D . PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori. Nucleic Acids Res. 1992; 20(23):6221-5. PMC: 334508. DOI: 10.1093/nar/20.23.6221. View

18.

Suerbaum S, Josenhans C, Labigne A . Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol. 1993; 175(11):3278-88. PMC: 204724. DOI: 10.1128/jb.175.11.3278-3288.1993. View

19.

Moore R, Kureishi A, Wong S, Bryan L . Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes. J Clin Microbiol. 1993; 31(5):1334-5. PMC: 262931. DOI: 10.1128/jcm.31.5.1334-1335.1993. View

20.

Desai M, Linton D, Owen R, Stanley J . Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease gene polymorphism. Epidemiol Infect. 1994; 112(1):151-60. PMC: 2271484. DOI: 10.1017/s0950268800057514. View