The Bcl-2/JH Gene Rearrangement is Undetectable in Hodgkin's Lymphomas: Results from the German Hodgkin Trial

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 1995 Jan 1

PMID 7704322

Citations 1

Authors

M Nolte

M Werner

W Spann

B Schnabel

R von Wasielewski

L Wilkens

K Hubner

R Fischer

A Georgii

Affiliations

Soon will be listed here.

Abstract

Ninety-one Hodgkin's lymphomas (HD), 52 non-Hodgkin lymphomas (NHL) and 33 specimens of non-neoplastic lymphatic tissues were investigated by polymerase chain reaction (PCR) for the presence of the bcl-2/JH gene rearrangement. The majority of the HD cases were drawn from the files of the German Hodgkin trial where diagnoses are established by a panel of four independent histopathologists. Using the very sensitive PCR method which detected 1 positive among 10000 negative cells, the bcl-2/JH gene rearrangement was found in 7/52 NHL and 3/16 tonsils with follicular hyperplasia, but in none of the 91 HD. The bcl-2 protein, however, was expressed by malignant cells of B and T cell lymphomas and by the giant tumour cells in 2/13 HD lymphocyte predominant, 11/28 HD nodular sclerosing I, 14/17 HD nodular sclerosing II, 10/27 HD mixed cellularity and 3/3 HD lymphocyte depleted. The bcl-2/JH rearrangement is thus independent of protein over-expression, the latter being found in all types of lymphomas. Our results do not confirm the findings of others who have detected the bcl-2/JH rearrangement in HD. These discrepancies may be explained by differences in choice of material, the gene rearrangement actually occurring in bystander cells but not in Reed-Sternberg or Hodgkin cells, or by contamination.

Citing Articles

Detection of bcl-2 and bax expression and bcl-2/JH fusion gene in intrahepatic cholangiocarcinoma.

Guo L, Xiao S, Guo Y World J Gastroenterol. 2004; 10(22):3251-4.

PMID: 15484294 PMC: 4572289. DOI: 10.3748/wjg.v10.i22.3251.

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