Brain-specific Tropomyosins TMBr-1 and TMBr-3 Have Distinct Patterns of Expression During Development and in Adult Brain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Nov 1

PMID 7694294

Citations 15

Authors

S Stamm

D Casper

J P Lees-Miller

D M Helfman

Affiliations

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Abstract

In this study we report on the developmental and regional expression of two brain-specific isoforms of tropomyosin, TMBr-1 and TMBr-3, that are generated from the rat alpha-tropomyosin gene via the use of alternative promoters and alternative RNA splicing. Western blot analysis using an exon-specific peptide polyclonal antibody revealed that the two isoforms are differentially expressed in development with TMBr-3 appearing in the embryonic brain at 16 days of gestation, followed by the expression of TMBr-1 at 20 days after birth. TMBr-3 was detected in all brain regions examined, whereas TMBr-1 was detected predominantly in brain areas that derived from the prosencephalon. Immunocytochemical studies on mixed primary cultures made from rat embryonic midbrain indicate that expression of the brain-specific epitope is restricted to neurons. The developmental pattern and neuronal localization of these forms of tropomyosin suggest that these isoforms have a specialized role in the development and plasticity of the nervous system.

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