Article: Classification of respiratory tract picornavirus isolates as enteroviruses or rhinoviruses by using reverse transcription-polymerase chain reaction

The classification of picornaviruses isolated from respiratory secretions as human rhinoviruses (HRVs) or enteroviruses (EVs) by using reverse transcription-polymerase chain reaction was compared to that derived from acid lability testing. Of the 135 clinical isolates examined, 91 were found to be HRVs and 44 were EVs. There was 100% concordance between the two classification methods. Reverse transcription-polymerase chain reaction is an effective alternative to traditional methods for differentiating HRVs from EVs.

Citing Articles

Distinctive clinical features of human bocavirus in children younger than 2 years.

Moriyama Y, Hamada H, Okada M, Tsuchiya N, Maru H, Shirato Y Eur J Pediatr. 2010; 169(9):1087-92.

PMID: 20383526 PMC: 2908446. DOI: 10.1007/s00431-010-1183-x.

Rapid detection and quantitation of poliovirus and rhinovirus sequences in viral stocks and infected cells.

Kotla S, Major S, Gustin K J Virol Methods. 2009; 157(1):32-9.

PMID: 19124042 PMC: 2698701. DOI: 10.1016/j.jviromet.2008.12.005.

Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses.

Lu X, Holloway B, Dare R, Kuypers J, Yagi S, Williams J J Clin Microbiol. 2007; 46(2):533-9.

PMID: 18057136 PMC: 2238069. DOI: 10.1128/JCM.01739-07.

Amplicon sequencing and improved detection of human rhinovirus in respiratory samples.

Deffernez C, Wunderli W, Thomas Y, Yerly S, Perrin L, Kaiser L J Clin Microbiol. 2004; 42(7):3212-8.

PMID: 15243084 PMC: 446277. DOI: 10.1128/JCM.42.7.3212-3218.2004.

Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays.

Coiras M, Aguilar J, Garcia M, Casas I, Perez-Brena P J Med Virol. 2004; 72(3):484-95.

PMID: 14748074 PMC: 7166637. DOI: 10.1002/jmv.20008.

References

1.

Kwok S, Higuchi R . Avoiding false positives with PCR. Nature. 1989; 339(6221):237-8. DOI: 10.1038/339237a0. View

2.

Petitjean J, Quibriac M, Freymuth F, Fuchs F, Laconche N, Aymard M . Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. J Clin Microbiol. 1990; 28(2):307-11. PMC: 269596. DOI: 10.1128/jcm.28.2.307-311.1990. View

3.

Olive D, Al-Mufti S, Khan M, Pasca A, Stanway G, Al-Nakib W . Detection and differentiation of picornaviruses in clinical samples following genomic amplification. J Gen Virol. 1990; 71 ( Pt 9):2141-7. DOI: 10.1099/0022-1317-71-9-2141. View

4.

Zoll G, Melchers W, KOPECKA H, Jambroes G, van der Poel H, Galama J . General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. J Clin Microbiol. 1992; 30(1):160-5. PMC: 265013. DOI: 10.1128/jcm.30.1.160-165.1992. View

5.

Abraham R, Chonmaitree T, McCombs J, Prabhakar B, Lo Verde P, Ogra P . Rapid detection of poliovirus by reverse transcription and polymerase chain amplification: application for differentiation between poliovirus and nonpoliovirus enteroviruses. J Clin Microbiol. 1993; 31(2):395-9. PMC: 262772. DOI: 10.1128/jcm.31.2.395-399.1993. View

Classification of Respiratory Tract Picornavirus Isolates As Enteroviruses or Rhinoviruses by Using Reverse Transcription-polymerase Chain Reaction