N-acetyl-D-glucosamine Medium Improves Recovery of Haemophilus Influenzae from Sputa of Patients with Cystic Fibrosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1993 Jul 1

PMID 7688756

Citations 2

Authors

L V Moller

L van Alphen

H Grasselier

J Dankert

Affiliations

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Abstract

A modified selective medium supplemented with N-acetyl-D-glucosamine (NAG), hemin, and NAD plus two cefsulodin disks, for primary isolation of nonencapsulated Haemophilus influenzae from sputum of patients with cystic fibrosis, is described. Isolation of H. influenzae from this medium, designated NAG medium, was compared with recovery by standard media and immunochemical detection of H. influenzae with monoclonal antibody 8BD9. The H. influenzae recovery rate increased from 31% with standard media to 42% with NAG medium. H. influenzae was detected by immunoperoxidase staining in 54% of the sputum specimens. The results of this study demonstrate that NAG medium improves H. influenzae recovery, although immunoperoxidase staining is superior for detection of H. influenzae from sputum of cystic fibrosis patients.

Citing Articles

Antimicrobial susceptibility of Haemophilus influenzae in the respiratory tracts of patients with cystic fibrosis.

Moller L, Regelink A, Grasselier H, van Alphen L, Dankert J Antimicrob Agents Chemother. 1998; 42(2):319-24.

PMID: 9527779 PMC: 105407. DOI: 10.1128/AAC.42.2.319.

Variation in metabolic enzyme activity of persistent Haemophilus influenzae in respiratory tracts of patients with cystic fibrosis.

Moller L, Grasselier H, Dankert J, van Alphen L J Clin Microbiol. 1996; 34(8):1926-9.

PMID: 8818883 PMC: 229155. DOI: 10.1128/jcm.34.8.1926-1929.1996.

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