A Comparison of the T Cell Delayed-type Hypersensitivity Epitopes of the 19-kD Antigens from Mycobacterium Tuberculosis and Myco. Intracellulare Using Overlapping Synthetic Peptides

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1993 Aug 1

PMID 7688674

Citations 8

Authors

J C Mackall

G H Bai

D A Rouse

G R Armoa

F Chuidian

J Nair

S L Morris

Affiliations

Soon will be listed here.

Abstract

Mycobacterial disease remains a serious international public health concern. Improved methods to rapidly and specifically detect mycobacterial infections would greatly enhance clinical management of these diseases. To define species-specific T cell epitopes that may be useful for the immunodiagnosis of mycobacterial infections, polymerized synthetic peptides from the 19-kD Mycobacterium tuberculosis and Myco. intracellulare protein homologues were tested in guinea pig DTH assays. Five Myco. tuberculosis and eight Myco. intracellulare peptides evoked skin test responses. Although all of the active Myco. tuberculosis and seven of the Myco. intracellulare peptides elicited non-specific DTH reactions, the peptide IN13 induced a Myco. intracellulare-specific skin test reaction, and thus represents a specific Myco. intracellulare T cell DTH epitope. This result suggests that the development of monospecific peptide-based immunodiagnostic reagents may be feasible for future clinical use.

Citing Articles

Identification of mimotopes of Mycobacterium leprae as potential diagnostic reagents.

Alban S, de Moura J, Minozzo J, Mira M, Soccol V BMC Infect Dis. 2013; 13:42.

PMID: 23351151 PMC: 3585472. DOI: 10.1186/1471-2334-13-42.

The determination of in vivo envelope-specific cell-mediated immune responses in equine infectious anemia virus-infected ponies.

Liu C, Cook F, Cook S, Craigo J, Even D, Issel C Vet Immunol Immunopathol. 2012; 148(3-4):302-10.

PMID: 22795699 PMC: 4337896. DOI: 10.1016/j.vetimm.2012.06.018.

Human T cell responses to peptides of the Mycobacterium leprae 45-kD serine-rich antigen.

Brahmbhatt S, Hussain R, Zafar S, Dawood G, Ottenhoff T, Drijfhout J Clin Exp Immunol. 2002; 128(1):140-8.

PMID: 11982601 PMC: 1906361. DOI: 10.1046/j.1365-2249.2002.01796.x.

A postgenomic approach to identification of Mycobacterium leprae-specific peptides as T-cell reagents.

Dockrell H, Brahmbhatt S, Robertson B, Britton S, Fruth U, Gebre N Infect Immun. 2000; 68(10):5846-55.

PMID: 10992494 PMC: 101546. DOI: 10.1128/IAI.68.10.5846-5855.2000.

Use of a whole blood assay to evaluate in vitro T cell responses to new leprosy skin test antigens in leprosy patients and healthy subjects.

Weir R, Brennan P, Butlin C, Dockrell H Clin Exp Immunol. 1999; 116(2):263-9.

PMID: 10337017 PMC: 1905275. DOI: 10.1046/j.1365-2249.1999.00892.x.

References

Chaparas S . Antigenic relationships among mycobacterial species studied by modified-rocket and crossed immunoelectrophoresis. Rev Infect Dis. 1981; 3(5):934-43. DOI: 10.1093/clinids/3.5.934. View

Nair J, Rouse D, Morris S . Nucleotide sequence analysis and serologic characterization of a 27-kilodalton Mycobacterium intracellulare lipoprotein. Infect Immun. 1993; 61(3):1074-81. PMC: 302841. DOI: 10.1128/iai.61.3.1074-1081.1993. View

Delisi C, Berzofsky J . T-cell antigenic sites tend to be amphipathic structures. Proc Natl Acad Sci U S A. 1985; 82(20):7048-52. PMC: 391307. DOI: 10.1073/pnas.82.20.7048. View

MINDEN P, Houghten R, Spear J, Shinnick T . A chemically synthesized peptide which elicits humoral and cellular immune responses to mycobacterial antigens. Infect Immun. 1986; 53(3):560-4. PMC: 260827. DOI: 10.1128/iai.53.3.560-564.1986. View

Oftung F, Mustafa A, Husson R, Young R, Godal T . Human T cell clones recognize two abundant Mycobacterium tuberculosis protein antigens expressed in Escherichia coli. J Immunol. 1987; 138(3):927-31. View

Rothbard J, Taylor W . A sequence pattern common to T cell epitopes. EMBO J. 1988; 7(1):93-100. PMC: 454220. DOI: 10.1002/j.1460-2075.1988.tb02787.x. View

Anderson D, van Schooten W, Barry M, Janson A, Buchanan T, de Vries R . A Mycobacterium leprae-specific human T cell epitope cross-reactive with an HLA-DR2 peptide. Science. 1988; 242(4876):259-61. DOI: 10.1126/science.2459778. View

. American Thoracic Society. Diagnostic standards and classification of tuberculosis. Am Rev Respir Dis. 1990; 142(3):725-35. DOI: 10.1164/ajrccm/142.3.725. View

Horsburgh Jr C . Mycobacterium avium complex infection in the acquired immunodeficiency syndrome. N Engl J Med. 1991; 324(19):1332-8. DOI: 10.1056/NEJM199105093241906. View

10.

Barnes P, Bloch A, Davidson P, Snider Jr D . Tuberculosis in patients with human immunodeficiency virus infection. N Engl J Med. 1991; 324(23):1644-50. DOI: 10.1056/NEJM199106063242307. View

11.

Andersen A, Ljungqvist L, Haslov K, Bentzon M . MPB 64 possesses 'tuberculosis-complex'-specific B- and T-cell epitopes. Scand J Immunol. 1991; 34(3):365-72. DOI: 10.1111/j.1365-3083.1991.tb01558.x. View

12.

Harris D, Vordermeier H, Roman E, Lathigra R, Brett S, Moreno C . Murine T cell-stimulatory peptides from the 19-kDa antigen of Mycobacterium tuberculosis. Epitope-restricted homology with the 28-kDa protein of Mycobacterium leprae. J Immunol. 1991; 147(8):2706-12. View

13.

Faith A, Moreno C, Lathigra R, Roman E, Fernandez M, Brett S . Analysis of human T-cell epitopes in the 19,000 MW antigen of Mycobacterium tuberculosis: influence of HLA-DR. Immunology. 1991; 74(1):1-7. PMC: 1384662. View

14.

Ashbridge K, Backstrom B, Liu H, Vikerfors T, Englebretsen D, Harding D . Mapping of T helper cell epitopes by using peptides spanning the 19-kDa protein of Mycobacterium tuberculosis. Evidence for unique and shared epitopes in the stimulation of antibody and delayed-type hypersensitivity responses. J Immunol. 1992; 148(7):2248-55. View

15.

Edlin B, Tokars J, GRIECO M, Crawford J, Williams J, Sordillo E . An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome. N Engl J Med. 1992; 326(23):1514-21. DOI: 10.1056/NEJM199206043262302. View

16.

Huebner R, Schein M, Cauthen G, Geiter L, SELIN M, Good R . Evaluation of the clinical usefulness of mycobacterial skin test antigens in adults with pulmonary mycobacterioses. Am Rev Respir Dis. 1992; 145(5):1160-6. DOI: 10.1164/ajrccm/145.5.1160. View

17.

Estrada I, Gutierrez M, Esparza J, Quesada-Pascual F, Estrada-Parra S, Possani L . Use of synthetic peptides corresponding to sequences of Mycobacterium leprae proteins to study delayed-type hypersensitivity response in sensitized guinea pigs. Int J Lepr Other Mycobact Dis. 1992; 60(1):18-27. View

18.

Vordermeier H, Harris D, Mehrotra P, Roman E, Elsaghier A, Moreno C . M. tuberculosis-complex specific T-cell stimulation and DTH reactions induced with a peptide from the 38-kDa protein. Scand J Immunol. 1992; 35(6):711-8. DOI: 10.1111/j.1365-3083.1992.tb02979.x. View

19.

Fischl M, Uttamchandani R, Daikos G, Poblete R, Moreno J, Reyes R . An outbreak of tuberculosis caused by multiple-drug-resistant tubercle bacilli among patients with HIV infection. Ann Intern Med. 1992; 117(3):177-83. DOI: 10.7326/0003-4819-117-3-177. View

20.

DOOLEY S, Jarvis W, Martone W, Snider Jr D . Multidrug-resistant tuberculosis. Ann Intern Med. 1992; 117(3):257-9. DOI: 10.7326/0003-4819-117-3-257. View