Determination in Situ of Neutral and Acidic Fucose-containing Oligosaccharides in the Bovine Submandibular Gland

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1993 Mar 1

PMID 7684035

Authors

G Menghi

D Accili

P Scocco

G Materazzi

Affiliations

Soon will be listed here.

Abstract

Lectins conjugated to horseradish peroxidase in combination with selected exoglycosidase digestion procedures were used to localize fucoglycoconjugates in the bovine submandibular gland. In particular, sequential treatments were employed to determine the distribution of neutral and acidic fucose-containing oligosaccharides that were previously shown to be present by biochemical techniques. Information was obtained on the distribution of the acidic oligosaccharide A-1a, alpha-Fuc(1-->2)-beta-Gal-(1-->4)-beta-GlcNAc-(1-->3)-[alpha-NeuAc-(2--> 6)]-GalNAc-ol, which was sequenced in situ and localized in acinar cells.

References

Menghi G, Accili D, Bondi A, Gabrielli M . Enzymatic degradation and quantitative lectin labeling for characterizing glycoconjugates which act as lectin acceptors in cat submandibular gland. Histochemistry. 1989; 90(5):331-8. DOI: 10.1007/BF00508309. View

Menghi G . Reactivity of peroxidase-labeled lectins in rabbit submandibular and sublingual glands. Acta Histochem. 1984; 75(1):27-35. DOI: 10.1016/S0065-1281(84)80069-0. View

Schulte B, SPICER S, Miller R . Lectin histochemistry of secretory and cell-surface glycoconjugates in the ovine submandibular gland. Cell Tissue Res. 1985; 240(1):57-66. DOI: 10.1007/BF00217558. View

Menghi G, Bondi A, Accili D, Fumagalli L, Materazzi G . Characterisation in situ of the complex carbohydrates in rabbit oviduct using digestion with glycosidases followed by lectin binding. J Anat. 1985; 140 ( Pt 4):613-25. PMC: 1165086. View

Ito N, Nishi K, Nakajima M, Okamura Y, Hirota T . Effects of alpha-galactosidase digestion on lectin staining in human pancreas. Histochemistry. 1988; 89(2):121-8. DOI: 10.1007/BF00489915. View

GRAHAM Jr R, Karnovsky M . The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966; 14(4):291-302. DOI: 10.1177/14.4.291. View

Menghi G, Ottaviani E, Accili D, Bolognani Fantin A . Identification of muramyl derivatives in Mollusca Gastropoda tissue. Histochemistry. 1991; 96(3):209-13. DOI: 10.1007/BF00271539. View

Tsuji T, Osawa T . Carbohydrate structures of bovine submaxillary mucin. Carbohydr Res. 1986; 151:391-402. DOI: 10.1016/s0008-6215(00)90358-6. View

DArcy S, Donoghue C, Koeleman C, van den Eijnden D, Savage A . Determination of the structure of a novel acidic oligosaccharide with blood-group activity isolated from bovine submaxillary-gland mucin. Biochem J. 1989; 260(2):389-93. PMC: 1138681. DOI: 10.1042/bj2600389. View

10.

Plendl J, SCHONLEBER B, Schmahl W, Schumacher U . Comparison of the unmasking of lectin receptors by neuraminidase and by enzyme-free buffer alone. J Histochem Cytochem. 1989; 37(11):1743-4. DOI: 10.1177/37.11.2809178. View

11.

Laden S, Schulte B, SPICER S . Histochemical evaluation of secretory glycoproteins in human salivary glands with lectin-horseradish peroxidase conjugates. J Histochem Cytochem. 1984; 32(9):965-72. DOI: 10.1177/32.9.6379041. View

12.

SPICER S, Schulte B . Detection and differentiation of glycoconjugates in various cell types by lectin histochemistry. Basic Appl Histochem. 1988; 32(3):307-20. View

13.

Fleming N, Brent M, Arellano R, Forstner J . Purification and immunofluorescent localization of rat submandibular mucin. Biochem J. 1982; 205(1):225-33. PMC: 1158467. DOI: 10.1042/bj2050225. View

14.

Menghi G, Bondi A, Materazzi G . Distribution of lectin binding sites in rabbit oviduct. Anat Rec. 1985; 211(3):279-84. DOI: 10.1002/ar.1092110308. View

15.

Bolognani Fantin A, Menghi G, Franchini A, Bondi A, Accili D, Fuhrman Conti A . Characterization of glycoconjugates in an embryonic human epithelial line and changes consequent to adaptation to a hyperosmotic medium. Histochem J. 1989; 21(2):79-88. DOI: 10.1007/BF01005983. View

16.

Schulte B, SPICER S . Histochemical methods for characterizing secretory and cell surface sialoglycoconjugates. J Histochem Cytochem. 1985; 33(5):427-38. DOI: 10.1177/33.5.3989272. View

17.

Schulte B, SPICER S . Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. II. Rat. Histochem J. 1984; 16(1):3-20. DOI: 10.1007/BF01003432. View

18.

Ito N, Nishi K, Nakajima M, Okamura Y, Hirota T . Histochemical analysis of the chemical structure of blood group-related carbohydrate chains in serous cells of human submandibular glands using lectin staining and glycosidase digestion. J Histochem Cytochem. 1989; 37(7):1115-24. DOI: 10.1177/37.7.2499620. View

19.

Ito N, Nishi K, Nakajima M, Okamura Y, Hirota T . Effects of alpha-L-fucosidase digestion on lectin staining in human pancreas. J Histochem Cytochem. 1988; 36(5):503-9. DOI: 10.1177/36.5.2451690. View

20.

Corfield A, Corfield C, Veh R, Wagner S, CLAMP J, Schauer R . Characterization of the major and minor mucus glycoproteins from bovine submandibular gland. Glycoconj J. 1991; 8(4):330-9. DOI: 10.1007/BF00731345. View