Expression of Hepatitis C Virus Core Protein As a Fusion Protein with Maltose Binding Protein. Detection of Anti-hepatitis C Core Antibody by Western Blot

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 1993 Apr 1

PMID 7681745

Citations 1

Authors

O Yokosuka

Y Ito

J Sakuma

F Imazeki

M OHTO

M Omata

Affiliations

Soon will be listed here.

Abstract

Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed in Escherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.

Citing Articles

Early detection of anti-HCc antibody in acute hepatitis C virus (HCV) by western blot (immunoblot) using a recombinant HCV core protein fragment.

Yeh C, Han C, Lo S, Ou J, Fan K, Sheen I J Clin Microbiol. 1994; 32(9):2235-41.

PMID: 7529251 PMC: 263974. DOI: 10.1128/jcm.32.9.2235-2241.1994.

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