Different Rates of HLA Class I Molecule Assembly Which Are Determined by Amino Acid Sequence in the Alpha 2 Domain

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1993 Jan 1

PMID 7678580

Citations 12

Authors

A Hill

M Takiguchi

A McMichael

Affiliations

Soon will be listed here.

Abstract

Assembly of HLA class I molecules was studied using pulse-chase labeling of B-lymphoblastoid cell lines with 35S-methionine, immunoprecipitation with antibodies detecting free or beta 2-microglobulin-associated heavy chain and isoelectric focusing. Marked differences between the products of different class I alleles were noted. HLA-B51 assembled very inefficiently, with considerable free heavy chain still detected in an unsialated form after a four hour chase. The closely related molecule HLA-B35 was in contrast rapidly assembled, all newly synthesized heavy chain being detected in a beta 2m-associated sialated form within 30 minutes. Analysis of naturally occurring variants related to HLA-B35 and HLA-B51 localized the region determining assembly efficiency to the alpha 2 domain, in which these molecules differ at eight amino acid residues. The effect was not due to a linked dominant gene, as both patterns of assembly were observed in a single cell line.

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