A Nuclear Mutation Defective in Mitochondrial Recombination in Yeast

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1995 Aug 15

PMID 7664749

Citations 36

Authors

F Ling

F Makishima

N Morishima

T Shibata

Affiliations

Soon will be listed here.

Abstract

Homologous recombination (crossing over and gene conversion) is generally essential for heritage and DNA repair, and occasionally causes DNA aberrations, in nuclei of eukaryotes. However, little is known about the roles of homologous recombination in the inheritance and stability of mitochondrial DNA which is continuously damaged by reactive oxygen species, by-products of respiration. Here, we report the first example of a nuclear recessive mutation which suggests an essential role for homologous recombination in the stable inheritance of mitochondrial DNA. For the detection of this class of mutants, we devised a novel procedure, 'mitochondrial crossing in haploid', which has enabled us to examine many mutant clones. Using this procedure, we examined mutants of Saccharomyces cerevisiae that showed an elevated UV induction of respiration-deficient mutations. We obtained a mutant that was defective in both the omega-intron homing and Endo.SceI-induced homologous gene conversion. We found that the mutant cells are temperature sensitive in the maintenance of mitochondrial DNA. A tetrad analysis indicated that elevated UV induction of respiration-deficient mutations, recombination deficiency and temperature sensitivity are all caused by a single nuclear mutation (mhr1) on chromosome XII. The pleiotropic characteristics of the mutant suggest an essential role for the MHR1 gene in DNA repair, recombination and the maintenance of DNA in mitochondria.

Citing Articles

Rolling-Circle Replication in Mitochondrial DNA Inheritance: Scientific Evidence and Significance from Yeast to Human Cells.

Ling F, Yoshida M Genes (Basel). 2020; 11(5).

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Evidence for the paternal mitochondrial DNA in the crucian carp-like fish lineage with hybrid origin.

Wang S, Jiao N, Zhao L, Zhang M, Zhou P, Huang X Sci China Life Sci. 2019; 63(1):102-115.

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Elucidating genomic patterns and recombination events in plant cybrid mitochondria.

Garcia L, Zubko M, Zubko E, Sanchez-Puerta M Plant Mol Biol. 2019; 100(4-5):433-450.

PMID: 30968307 DOI: 10.1007/s11103-019-00869-z.

Prevention of mitochondrial genomic instability in yeast by the mitochondrial recombinase Mhr1.

Ling F, Bradshaw E, Yoshida M Sci Rep. 2019; 9(1):5433.

PMID: 30931958 PMC: 6443803. DOI: 10.1038/s41598-019-41699-9.

Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.

Prasai K, Robinson L, Tatchell K, Harrison L Mitochondrion. 2017; 42:23-32.

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