Determination of Heteronuclear Three-bond J-coupling Constants in Peptides by a Simple Heteronuclear Relayed E.COSY Experiment

Overview

Journal J Biomol NMR

Publisher Springer

Specialties Molecular Biology
Nuclear Medicine

Date 1995 Jul 1

PMID 7663145

Citations 4

Authors

J M Schmidt

R R Ernst

S Aimoto

M Kainosho

Affiliations

Soon will be listed here.

Abstract

A simple heteronuclear relayed E.COSY pulse sequence with a minimum number of pulses is proposed for the quantitative determination of heteronuclear three-bond J-coupling constants in uniformly 13C-enriched polypeptide samples. Numerous heteronuclear three-bond coupling constants, including 3JHNC, 3JHNC beta, 3JH beta C, and 3JH alpha C gamma, can be determined for each residue from a single heteronuclear relayed E.COSY spectrum. Couplings relevant for stereospecific assignments as well as for the determination of dihedral angles in the amino acid backbone and in side chains are obtained. The method is demonstrated on the uniformly 13C-enriched decapeptide antamanide (-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9-Phe1 0-).

Citing Articles

Self-consistent 3J coupling analysis for the joint calibration of Karplus coefficients and evaluation of torsion angles.

Schmidt J, Blumel M, Lohr F, Ruterjans H J Biomol NMR. 2010; 14(1):1-12.

PMID: 21136331 DOI: 10.1023/A:1008345303942.

Alternative E.COSY techniques for the measurement of 3J(C (i) (') (-1),C (i) (beta) ) and (3) J(H (i) (N) ,C (i) (beta) ) coupling constants in proteins.

Lohr F, Ruterjans H J Biomol NMR. 2010; 13(3):263-74.

PMID: 20700819 DOI: 10.1023/A:1008378719908.

Heteronuclear relayed E.COSY revisited: determination of 3J(H(alpha),C(gamma)) couplings in Asx and aromatic residues in proteins.

Lohr F, Perez C, Kohler R, Ruterjans H, Schmidt J J Biomol NMR. 2000; 18(1):13-22.

PMID: 11061224 DOI: 10.1023/a:1008385202639.

Heteronuclear relayed E.COSY applied to the determination of accurate 3J(HN,C') and 3J(H beta,C') coupling constants in desulfovibrio vulgaris flavodoxin.

Schmidt J, Lohr F, Ruterjans H J Biomol NMR. 1996; 7(2):142-52.

PMID: 8616270 DOI: 10.1007/BF00203824.

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