Heterogeneity of Rice Ragged Stunt Oryzavirus Genome Segment 9 and Its Segregation by Insect Vector Transmission

Overview

Journal Arch Virol

Specialty Microbiology

Date 1995 Jan 1

PMID 7661702

Authors

H Suga

I UYEDA

J Yan

K Murao

I Kimura

E R Tiongco

P Cabautan

H Koganezawa

Affiliations

Soon will be listed here.

Abstract

Genomic heterogeneity of genome segment 9 (S9) of rice ragged stunt virus (RRSV) was investigated and a point mutation was found to be responsible for an electrophoretic mobility shift of S9 on polyacrylamide gel electrophoresis (PAGE). A new form of S9 (S9L) which migrated slightly faster than natural S9 (S9U) had the same length with A-->C transversion at nt 843. Synthetic S9 with a C:G pair at nt 843 migrated slightly faster than that with an A:U pair. Therefore, we conclude that the single point mutation shifts the electrophoretic mobility. Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), we could detect S9U and S9L alone or mixture in insect vectors after acquisition as well as in infected rice plants.

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