Temporal, 3-dimensional, Cellular Anatomy of Corneal Wound Tissue

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 1995 Apr 1

PMID 7649828

Citations 23

Authors

J V Jester

W M Petroll

P A Barry

H D Cavanagh

Affiliations

Soon will be listed here.

Abstract

We have evaluated temporally the 3-dimensional cellular anatomy of corneal wound tissue in the rabbit eye using in vivo tandem scanning confocal microscopy. In vivo microscopic studies showed that corneal fibroblast migrated into the wound as an interconnected cellular meshwork with long, thin, randomly oriented cell processes. Interconnection of fibroblasts was further confirmed by localisation of monoclonal antibodies to connexin 43 which demonstrated prominent staining of putative gap junctions between fibroblasts. Temporal observations indicated that the interconnected cells and cellular processes undergo sequential positional changes leading to orientation of cells and interconnected cell processes parallel to the wound margin. Laser scanning confocal microscopy of en bloc, phalloidin-stained corneal wounds showed prominent intracellular f-actin bundles (i.e. stress fibres) within cell processes which formed an extensive interwoven pattern within the wound.

Citing Articles

The impact of UV cross-linking on corneal stromal cell migration, differentiation and patterning.

Petroll W, Miron-Mendoza M, Sunkara Y, Ikebe H, Sripathi N, Hassaniardekani H Exp Eye Res. 2023; 233:109523.

PMID: 37271309 PMC: 10825899. DOI: 10.1016/j.exer.2023.109523.

Keratocyte mechanobiology.

Petroll W, Varner V, Schmidtke D Exp Eye Res. 2020; 200:108228.

PMID: 32919993 PMC: 7655662. DOI: 10.1016/j.exer.2020.108228.

Intrastromal Injection of Hyaluronidase Alters the Structural and Biomechanical Properties of the Corneal Stroma.

Kim S, Jalilian I, Thomasy S, Bowman M, Raghunathan V, Song Y Transl Vis Sci Technol. 2020; 9(6):21.

PMID: 32821518 PMC: 7409307. DOI: 10.1167/tvst.9.6.21.

Coupling of Fibrin Reorganization and Fibronectin Patterning by Corneal Fibroblasts in Response to PDGF BB and TGFβ1.

Miron-Mendoza M, Vazquez D, Garcia-Ramila N, Ikebe H, Petroll W Bioengineering (Basel). 2020; 7(3).

PMID: 32784578 PMC: 7552779. DOI: 10.3390/bioengineering7030089.

Cellular morphological changes detected by laser scanning in vivo confocal microscopy associated with clinical outcome in fungal keratitis.

Chidambaram J, Prajna N, Palepu S, Lanjewar S, Shah M, Elakkiya S Sci Rep. 2019; 9(1):8334.

PMID: 31171825 PMC: 6554396. DOI: 10.1038/s41598-019-44833-9.

References

Jester J, Petroll W, Garana R, Lemp M, Cavanagh H . Comparison of in vivo and ex vivo cellular structure in rabbit eyes detected by tandem scanning microscopy. J Microsc. 1992; 165(Pt 1):169-81. DOI: 10.1111/j.1365-2818.1992.tb04314.x. View

Rudolph R, McClure W, Woodward M . Contractile fibroblasts in chronic alcoholic cirrhosis. Gastroenterology. 1979; 76(4):704-9. View

Petroll W, Cavanagh H, Jester J . Three-dimensional imaging of corneal cells using in vivo confocal microscopy. J Microsc. 1993; 170(Pt 3):213-9. DOI: 10.1111/j.1365-2818.1993.tb03344.x. View

Watts G, Grillo H, Gross J . Studies in Wound Healing: II. The Role of Granulation Tissue in Contraction. Ann Surg. 1958; 148(2):153-60. PMC: 1450785. DOI: 10.1097/00000658-195808000-00002. View

Stopak D, Harris A . Connective tissue morphogenesis by fibroblast traction. I. Tissue culture observations. Dev Biol. 1982; 90(2):383-98. DOI: 10.1016/0012-1606(82)90388-8. View

Petroll W, Cavanagh H, Lemp M, ANDREWS P, Jester J . Digital image acquisition in in vivo confocal microscopy. J Microsc. 1992; 165(Pt 1):61-9. DOI: 10.1111/j.1365-2818.1992.tb04305.x. View

Gabbiani G, Chaponnier C, Huttner I . Cytoplasmic filaments and gap junctions in epithelial cells and myofibroblasts during wound healing. J Cell Biol. 1978; 76(3):561-8. PMC: 2110008. DOI: 10.1083/jcb.76.3.561. View

Petroll W, Cavanagh H, Barry P, Andrews P, Jester J . Quantitative analysis of stress fiber orientation during corneal wound contraction. J Cell Sci. 1993; 104 ( Pt 2):353-63. DOI: 10.1242/jcs.104.2.353. View

Jester J, ANDREWS P, Petroll W, Lemp M, Cavanagh H . In vivo, real-time confocal imaging. J Electron Microsc Tech. 1991; 18(1):50-60. DOI: 10.1002/jemt.1060180108. View

10.

Miller S, Garfield R, Daniel E . Improved propagation in myometrium associated with gap junctions during parturition. Am J Physiol. 1989; 256(1 Pt 1):C130-41. DOI: 10.1152/ajpcell.1989.256.1.C130. View

11.

Jester J, Rodrigues M, Herman I . Characterization of avascular corneal wound healing fibroblasts. New insights into the myofibroblast. Am J Pathol. 1987; 127(1):140-8. PMC: 1899587. View

12.

MARTIN P, Lewis J . Actin cables and epidermal movement in embryonic wound healing. Nature. 1992; 360(6400):179-83. DOI: 10.1038/360179a0. View

13.

Welch M, ODLAND G, Clark R . Temporal relationships of F-actin bundle formation, collagen and fibronectin matrix assembly, and fibronectin receptor expression to wound contraction. J Cell Biol. 1990; 110(1):133-45. PMC: 2115975. DOI: 10.1083/jcb.110.1.133. View

14.

Ehrlich H, Rajaratnam J . Cell locomotion forces versus cell contraction forces for collagen lattice contraction: an in vitro model of wound contraction. Tissue Cell. 1990; 22(4):407-17. DOI: 10.1016/0040-8166(90)90070-p. View

15.

Nagle R, Kneiser M, Bulger R, BENDITT E . Induction of smooth muscle characteristics in renal interstitial fibroblasts during obstructive nephropathy. Lab Invest. 1973; 29(4):422-7. View

16.

Darby I, Skalli O, Gabbiani G . Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab Invest. 1990; 63(1):21-9. View

17.

MacKenzie L, Garfield R . Hormonal control of gap junctions in the myometrium. Am J Physiol. 1985; 248(3 Pt 1):C296-308. DOI: 10.1152/ajpcell.1985.248.3.C296. View

18.

Jester J, Barry P, Lind G, Petroll W, Garana R, Cavanagh H . Corneal keratocytes: in situ and in vitro organization of cytoskeletal contractile proteins. Invest Ophthalmol Vis Sci. 1994; 35(2):730-43. View

19.

Petroll W, New K, Sachdev M, Cavanagh H, Jester J . Radial keratotomy. III. Relationship between wound gape and corneal curvature in primate eyes. Invest Ophthalmol Vis Sci. 1992; 33(12):3283-91. View

20.

Majno G, Gabbiani G, Hirschel B, RYAN G, Statkov P . Contraction of granulation tissue in vitro: similarity to smooth muscle. Science. 1971; 173(3996):548-50. DOI: 10.1126/science.173.3996.548. View