Ultrastructural Distribution of Endogenous Immunoglobulin-G in Human Term Amniochorion

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1995 Aug 1

PMID 7648630

Authors

N A Bright

C D Ockleford

Affiliations

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Abstract

Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible non-specific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the presence of small quantities of maternal IgG in the amniotic fluid.

References

Hay F, Hull M, Torrigiani G . The transfer of human IgG subclasses from mother to foetus. Clin Exp Immunol. 1971; 9(3):355-8. PMC: 1713070. View

Wynn R, French G . Comparative ultrastructure of the mammalian amnion. Obstet Gynecol. 1968; 31(6):759-74. View

Stiehm E, Fudenberg H . Serum levels of immune globulins in health and disease: a survey. Pediatrics. 1966; 37(5):715-27. View

Hindocha P, Wood C . The placental transfer of IgG subclasses in human pregnancy. Clin Exp Immunol. 1980; 41(2):303-8. PMC: 1537014. View

Tokuyasu K . A study of positive staining of ultrathin frozen sections. J Ultrastruct Res. 1978; 63(3):287-307. DOI: 10.1016/s0022-5320(78)80053-7. View

Bourne G . The microscopic anatomy of the human amnion and chorion. Am J Obstet Gynecol. 1960; 79:1070-3. DOI: 10.1016/0002-9378(60)90512-3. View

Gadow E, Floriani F, Florin A . IgG levels in amniotic fluid. Am J Obstet Gynecol. 1974; 119(6):849-51. DOI: 10.1016/0002-9378(74)90100-8. View

Einhorn M, Granoff D, Nahm M, Quinn A, Shackelford P . Concentrations of antibodies in paired maternal and infant sera: relationship to IgG subclass. J Pediatr. 1987; 111(5):783-8. DOI: 10.1016/s0022-3476(87)80268-8. View

Bright N, Ockleford C, Anwar M . Ontogeny and distribution of Fc gamma receptors in the human placenta. Transport or immune surveillance?. J Anat. 1994; 184 ( Pt 2):297-308. PMC: 1259990. View

10.

Curl C . Immunoglobulin levels in amniotic fluid. Am J Obstet Gynecol. 1971; 109(3):408-10. DOI: 10.1016/0002-9378(71)90336-x. View

11.

Wang A, Faulk W, Stuckey M, Fudenberg H . Chemical differences of adult, fetal and hypogammaglobulinemic IgG immunoglobulins. Immunochemistry. 1970; 7(8):703-8. DOI: 10.1016/0019-2791(70)90176-x. View

12.

ALLANSMITH M, Buell D . THE RELATIONSHIP OF GAMMA-1A GLOBULIN AND REAGIN IN CORD SERA. J Allergy. 1964; 35:339-45. DOI: 10.1016/0021-8707(64)90097-8. View

13.

Lanzavecchia G, Morano E . [Ultrastructure of the human amnion epithelium studied by electron microscope]. Arch Ital Anat Istol Patol. 1959; 64(1):74-86. View

14.

Monif G, MENDENHALL H . Immunoglobulin G levels and the titers of specific antiviral antibodies in amniotic fluid. Am J Obstet Gynecol. 1970; 108(4):651-4. DOI: 10.1016/0002-9378(70)90247-4. View

15.

Usategui-Gomez M, Stearns S . Comparative study of the Rh-D antibody titres of amniotic fluids and corresponding maternal sera in Rh-D sensitized pregnancies. Nature. 1969; 221(5175):82-3. DOI: 10.1038/221082a0. View

16.

Schmidt W . The amniotic fluid compartment: the fetal habitat. Adv Anat Embryol Cell Biol. 1992; 127:1-100. DOI: 10.1007/978-3-642-77300-6. View

17.

Virella G, Silveira Nunes M, Tamagnini G . Placental transfer of human IgG subclasses. Clin Exp Immunol. 1972; 10(3):475-8. PMC: 1713149. View

18.

Bright N, Ockleford C . Heterogeneity of Fc gamma receptor-bearing cells in human term amniochorion. Placenta. 1994; 15(3):247-55. DOI: 10.1016/0143-4004(94)90016-7. View

19.

Leslie G, Swate T . Structure and biologic functions of human IgD. I. The presence of immunoglobulin D in human cord sera. J Immunol. 1972; 109(1):47-50. View

20.

WASZ-HOCKERT O, Wager O, Hautala T, WIDHOLM O . Transmission of antibodies from mother to fetus; a study of the diphtheria antitoxin level in the newborn with oesophageal atresia. Ann Med Exp Biol Fenn. 1956; 34(4):444-6. View