Immunolocalization of the Neural Cell Adhesion Molecule L1 in Non-proliferating Epithelial Cells of the Male Urogenital Tract

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 1995 Apr 1

PMID 7648407

Citations 10

Authors

R Kujat

F Miragall

D Krause

R Dermietzel

K H Wrobel

Affiliations

Soon will be listed here.

Abstract

The localization of the neural cell adhesion molecule L1 in the male urogenital tract (including seminal vesicles and prostate) of the mouse and bull was investigated using immunocytochemical and immunochemical methods in order to better understand the function of this glycoprotein in non-neural tissues. L1 antibodies labeled non-myelinated nerves in all portions of the urogenital tract investigated. However, L1 immunoreactivity was also found between epithelial cells of several regions of the urogenital system including epididymal tail, deferent duct, ejaculatory duct and seminal vesicles. Some L1 immunoreactivity was also demonstrated between epithelial cells of murine urinary bladder and urethra. The specificity of the immunoreaction was verified by western blots. There was no correlation between L1 expression and proliferating activity as revealed by double immunocytochemistry using various markers of cell proliferation. This unexpected expression of L1 in nonneural tissues is mainly restricted to non-proliferating epithelia of those portions of the urogenital tract that are derived from the Wolffian duct. It is suggested that L1 in these epithelia could enhance the mechanical resistance and reduce transepithelial permeability.

Citing Articles

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Involvement of integrin alpha(v)beta(3) and cell adhesion molecule L1 in transendothelial migration of melanoma cells.

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Plasmin-sensitive dibasic sequences in the third fibronectin-like domain of L1-cell adhesion molecule (CAM) facilitate homomultimerization and concomitant integrin recruitment.

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References

Fischer G, Kunemund V, Schachner M . Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1. J Neurosci. 1986; 6(2):605-12. PMC: 6568534. View

Miragall F, Kadmon G, Husmann M, Schachner M . Expression of cell adhesion molecules in the olfactory system of the adult mouse: presence of the embryonic form of N-CAM. Dev Biol. 1988; 129(2):516-31. DOI: 10.1016/0012-1606(88)90397-1. View

Rathjen F, Schachner M . Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion. EMBO J. 1984; 3(1):1-10. PMC: 557289. DOI: 10.1002/j.1460-2075.1984.tb01753.x. View

Cunningham B, Hemperly J, Murray B, Prediger E, Brackenbury R, Edelman G . Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science. 1987; 236(4803):799-806. DOI: 10.1126/science.3576199. View

Miragall F, Kadmon G, Schachner M . Expression of L1 and N-CAM cell adhesion molecules during development of the mouse olfactory system. Dev Biol. 1989; 135(2):272-86. DOI: 10.1016/0012-1606(89)90179-6. View

Grumet M, Hoffman S, Edelman G . Two antigenically related neuronal cell adhesion molecules of different specificities mediate neuron-neuron and neuron-glia adhesion. Proc Natl Acad Sci U S A. 1984; 81(1):267-71. PMC: 344653. DOI: 10.1073/pnas.81.1.267. View

Wrobel K, Sinowatz F, Kugler P . [The functional morphology of the rete testis. tubuli recti and terminal segments of the semeniferous tubules in the mature bull]. Anat Histol Embryol. 1978; 7(4):320-35. DOI: 10.1111/j.1439-0264.1978.tb00671.x. View

Martini R, Schachner M . Immunoelectron microscopic localization of neural cell adhesion molecules (L1, N-CAM, and myelin-associated glycoprotein) in regenerating adult mouse sciatic nerve. J Cell Biol. 1988; 106(5):1735-46. PMC: 2115039. DOI: 10.1083/jcb.106.5.1735. View

Hsu S, RAINE L, FANGER H . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-80. DOI: 10.1177/29.4.6166661. View

10.

Lindner J, Rathjen F, Schachner M . L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum. Nature. 1983; 305(5933):427-30. DOI: 10.1038/305427a0. View

11.

Dermietzel R, Yancey S, Traub O, Willecke K, REVEL J . Major loss of the 28-kD protein of gap junction in proliferating hepatocytes. J Cell Biol. 1987; 105(4):1925-34. PMC: 2114657. DOI: 10.1083/jcb.105.4.1925. View

12.

Nieke J, Schachner M . Expression of the neural cell adhesion molecules L1 and N-CAM and their common carbohydrate epitope L2/HNK-1 during development and after transection of the mouse sciatic nerve. Differentiation. 1985; 30(2):141-51. DOI: 10.1111/j.1432-0436.1985.tb00525.x. View

13.

Miragall F, Kadmon G, Faissner A, Antonicek H, Schachner M . Retention of J1/tenascin and the polysialylated form of the neural cell adhesion molecule (N-CAM) in the adult olfactory bulb. J Neurocytol. 1990; 19(6):899-914. DOI: 10.1007/BF01186818. View

14.

Moos M, Tacke R, Scherer H, Teplow D, Fruh K, Schachner M . Neural adhesion molecule L1 as a member of the immunoglobulin superfamily with binding domains similar to fibronectin. Nature. 1988; 334(6184):701-3. DOI: 10.1038/334701a0. View

15.

Persohn E, Schachner M . Immunoelectron microscopic localization of the neural cell adhesion molecules L1 and N-CAM during postnatal development of the mouse cerebellum. J Cell Biol. 1987; 105(1):569-76. PMC: 2114902. DOI: 10.1083/jcb.105.1.569. View

16.

Bartsch U, Kirchhoff F, Schachner M . Immunohistological localization of the adhesion molecules L1, N-CAM, and MAG in the developing and adult optic nerve of mice. J Comp Neurol. 1989; 284(3):451-62. DOI: 10.1002/cne.902840310. View

17.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

18.

Nolte C, Martini R . Immunocytochemical localization of the L1 and N-CAM cell adhesion molecules and their shared carbohydrate epitope L2/HNK-1 in the developing and differentiated gustatory papillae of the mouse tongue. J Neurocytol. 1992; 21(1):19-33. DOI: 10.1007/BF01206895. View

19.

Chang S, Rathjen F, Raper J . Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins. J Cell Biol. 1987; 104(2):355-62. PMC: 2114414. DOI: 10.1083/jcb.104.2.355. View

20.

Takeichi M . The cadherins: cell-cell adhesion molecules controlling animal morphogenesis. Development. 1988; 102(4):639-55. DOI: 10.1242/dev.102.4.639. View