Immunocytochemistry and in Situ Hybridization in the Electron Microscope: Combined Application in the Study of Virus-infected Cells

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 1995 May 1

PMID 7641067

Citations 9

Authors

K Bienz

D Egger

Affiliations

Soon will be listed here.

Abstract

The present review evaluates methods for electron microscopic immunocytochemistry and in situ hybridization, using post-embedding techniques and colloidal gold as a label. Special emphasis is given to double labeling immunocytochemistry and double in situ hybridization and to their combined application on the same specimen. Brief guidelines are presented for fixation, embedding media, the use of polyclonal and monoclonal antibodies and nucleic acid probes. Conditions for labeling and binding of antibody and nucleic acid probes to the target and protocols for direct and indirect immunodetection are discussed. Combinations of direct and indirect immunodetections in multiple labeling experiments are summarized.

Citing Articles

Wrapping things up about virus RNA replication.

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PMID: 16190978 PMC: 7169867. DOI: 10.1111/j.1600-0854.2005.00339.x.

Replication complex of human parechovirus 1.

Krogerus C, Egger D, Samuilova O, Hyypia T, Bienz K J Virol. 2003; 77(15):8512-23.

PMID: 12857920 PMC: 165257. DOI: 10.1128/jvi.77.15.8512-8523.2003.

Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons.

Gosert R, Egger D, Lohmann V, Bartenschlager R, Blum H, Bienz K J Virol. 2003; 77(9):5487-92.

PMID: 12692249 PMC: 153965. DOI: 10.1128/jvi.77.9.5487-5492.2003.

Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex.

Egger D, Wolk B, Gosert R, Bianchi L, Blum H, Moradpour D J Virol. 2002; 76(12):5974-84.

PMID: 12021330 PMC: 136238. DOI: 10.1128/jvi.76.12.5974-5984.2002.

RNA replication of mouse hepatitis virus takes place at double-membrane vesicles.

Gosert R, Kanjanahaluethai A, Egger D, Bienz K, Baker S J Virol. 2002; 76(8):3697-708.

PMID: 11907209 PMC: 136101. DOI: 10.1128/jvi.76.8.3697-3708.2002.

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