Phototaxis Away from Blue Light by an Escherichia Coli Mutant Accumulating Protoporphyrin IX

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1995 Aug 1

PMID 7638191

Citations 28

Authors

H Yang

H Inokuchi

J Adler

Affiliations

Soon will be listed here.

Abstract

The hemH gene of Escherichia coli encodes ferrochelatase (EC 4.99.1.1), the enzyme that catalyzes the last step in the production of heme, namely the synthesis of heme from protoporphyrin IX plus Fe2+. The behavioral responses to light were studied in E. coli carrying a hemH mutation. It was shown that the hemH mutant displayed a tumbling response upon illumination and a running response upon removal of the light. The most effect light to induce a tumbling response in the hemH mutant was blue light (396-450 nm). The chemotaxis machinery was needed for the light-induced tumbling response in the hemH mutant. The bacterial defect is an analog of the human inherited disease erythropoietic protoporphyria.

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References

Burchard R, Gordon S, Dworkin M . Action spectrum for the photolysis of Myxococcus xanthus. J Bacteriol. 1965; 91(2):896-7. PMC: 314944. DOI: 10.1128/jb.91.2.896-897.1966. View

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

Armstrong J, Adler J, Dahl M . Nonchemotactic mutants of Escherichia coli. J Bacteriol. 1967; 93(1):390-8. PMC: 315011. DOI: 10.1128/jb.93.1.390-398.1967. View

Hazelbauer G, Mesibov R, Adler J . Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc Natl Acad Sci U S A. 1969; 64(4):1300-7. PMC: 223283. DOI: 10.1073/pnas.64.4.1300. View

Macnab R, Koshland Jr D . The gradient-sensing mechanism in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1972; 69(9):2509-12. PMC: 426976. DOI: 10.1073/pnas.69.9.2509. View

Mesibov R, Adler J . Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972; 112(1):315-26. PMC: 251414. DOI: 10.1128/jb.112.1.315-326.1972. View

Berg H, Brown D . Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972; 239(5374):500-4. DOI: 10.1038/239500a0. View

Cox R, CHARLES H . Porphyrin-accumulating mutants of Escherichia coli. J Bacteriol. 1973; 113(1):122-32. PMC: 251610. DOI: 10.1128/jb.113.1.122-132.1973. View

Tsang N, Macnab R, Koshland Jr D . Common mechanism for repellents and attractants in bacterial chemotaxis. Science. 1973; 181(4094):60-3. DOI: 10.1126/science.181.4094.60. View

10.

Tso W, Adler J . Negative chemotaxis in Escherichia coli. J Bacteriol. 1974; 118(2):560-76. PMC: 246789. DOI: 10.1128/jb.118.2.560-576.1974. View

11.

Larsen S, Reader R, Kort E, Tso W, Adler J . Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli. Nature. 1974; 249(452):74-7. DOI: 10.1038/249074a0. View

12.

Taylor B, Koshland Jr D . Intrinsic and extrinsic light responses of Salmonella typhimurium and Escherichia coli. J Bacteriol. 1975; 123(2):557-69. PMC: 235761. DOI: 10.1128/jb.123.2.557-569.1975. View

13.

Macnab R, Koshland Jr D . Bacterial motility and chemotaxis: light-induced tumbling response and visualization of individual flagella. J Mol Biol. 1974; 84(3):399-406. DOI: 10.1016/0022-2836(74)90448-3. View

14.

Springer M, Kort E, Larsen S, Ordal G, Reader R, Adler J . Role of methionine in bacterial chemotaxis: requirement for tumbling and involvement in information processing. Proc Natl Acad Sci U S A. 1975; 72(11):4640-4. PMC: 388779. DOI: 10.1073/pnas.72.11.4640. View

15.

Taylor B, Koshland Jr D . Perturbation of the chemotactic tumbling of bacteria. J Supramol Struct. 1976; 4(3):343-53. DOI: 10.1002/jss.400040305. View

16.

Miller J, Koshland Jr D . Effects of cyanine dye membrane probes on cellular properties. Nature. 1978; 272(5648):83-4. DOI: 10.1038/272083a0. View

17.

Kondoh H, Ball C, Adler J . Identification of a methyl-accepting chemotaxis protein for the ribose and galactose chemoreceptors of Escherichia coli. Proc Natl Acad Sci U S A. 1979; 76(1):260-4. PMC: 382918. DOI: 10.1073/pnas.76.1.260. View

18.

Taylor B, Miller J, Warrick H, Koshland Jr D . Electron acceptor taxis and blue light effect on bacterial chemotaxis. J Bacteriol. 1979; 140(2):567-73. PMC: 216683. DOI: 10.1128/jb.140.2.567-573.1979. View

19.

Szupica C, Adler J . Cell envelopes of chemotaxis mutants of Escherichia coli rotate their flagella counterclockwise. J Bacteriol. 1985; 162(1):451-3. PMC: 219013. DOI: 10.1128/jb.162.1.451-453.1985. View

20.

Slocum M, PARKINSON J . Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: null phenotypes of the tar and tap genes. J Bacteriol. 1985; 163(2):586-94. PMC: 219162. DOI: 10.1128/jb.163.2.586-594.1985. View