Fluorescent Dye-primer Cycle Sequencing Using Unpurified PCR Products As Templates; Development of a Protocol Amenable to High-throughput DNA Sequencing

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1995 Jun 25

PMID 7610069

Citations 5

Authors

M K Trower

D Burt

I J Purvis

C W Dykes

C Christodoulou

Affiliations

Soon will be listed here.

Citing Articles

On-line integration of PCR and cycle sequencing in capillaries: from human genomic DNA directly to called bases.

Hashimoto M, He Y, Yeung E Nucleic Acids Res. 2003; 31(8):e41.

PMID: 12682376 PMC: 153753. DOI: 10.1093/nar/gng041.

Thermal cycle dideoxy DNA sequencing.

Slatko B Mol Biotechnol. 1996; 6(3):311-22.

PMID: 9067976 DOI: 10.1007/BF02761709.

Ordered shotgun sequencing of a 135 kb Xq25 YAC containing ANT2 and four possible genes, including three confirmed by EST matches.

Chen C, Su Y, Baybayan P, Siruno A, Nagaraja R, Mazzarella R Nucleic Acids Res. 1996; 24(20):4034-41.

PMID: 8918809 PMC: 146183. DOI: 10.1093/nar/24.20.4034.

Molecule by molecule PCR amplification of complex DNA mixtures for direct sequencing: an approach to in vitro cloning.

Lukyanov K, Matz M, Bogdanova E, Gurskaya N, Lukyanov S Nucleic Acids Res. 1996; 24(11):2194-5.

PMID: 8668555 PMC: 145914. DOI: 10.1093/nar/24.11.2194.

Conservation of synteny between the genome of the pufferfish (Fugu rubripes) and the region on human chromosome 14 (14q24.3) associated with familial Alzheimer disease (AD3 locus).

Trower M, Orton S, Purvis I, Sanseau P, Riley J, Christodoulou C Proc Natl Acad Sci U S A. 1996; 93(4):1366-9.

PMID: 8643637 PMC: 39943. DOI: 10.1073/pnas.93.4.1366.

References

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Dowton M, Austin A . Direct sequencing of double-stranded PCR products without intermediate fragment purification; digestion with mung bean nuclease. Nucleic Acids Res. 1993; 21(15):3599-600. PMC: 331479. DOI: 10.1093/nar/21.15.3599. View

Wilson R . High-throughput purification of M13 templates for DNA sequencing. Biotechniques. 1993; 15(3):414-6, 418-20, 422. View

Hanke M, Wink M . Direct DNA sequencing of PCR-amplified vector inserts following enzymatic degradation of primer and dNTPs. Biotechniques. 1994; 17(5):858-60. View