Detection of Platelet-associated Antibodies by Flow Cytometry in Hematological Autoimmune Disorders

Overview

Journal Ann Hematol

Specialty Hematology

Date 1995 May 1

PMID 7599288

Citations 1

Authors

P L Tazzari

F Ricci

N Vianelli

C Tassi

D Belletti

I Pierri

L Gugliotta

M Gobbi

R Conte

Affiliations

Soon will be listed here.

Abstract

We describe our experience in the evaluation of platelet-associated immunoglobulins (PAIg) by flow cytometry in comparison to solid-phase assay in patients affected by idiopathic thrombocytopenic purpura and by Evans syndrome. Results show that the analysis of PAIg by flow cytometry is easy and reliable and correlates well with data obtained by the solid-phase technique. In addition, flow cytometry allows the evaluation of samples containing small numbers of platelets (< 20,000/mm3); the analysis is objective, not influenced by personal experience. Moreover, flow cytometry appears simple enough to be performed in a routine laboratory, and data might be retrieved to perform batch analysis. Our results appear to indicate that PAIg flow cytometry might be a sensitive tool for the evaluation of patients with autoimmune thrombocytopenia.

Citing Articles

Thrombocytopenic purpura as adverse reaction to recombinant hepatitis B vaccine.

Ronchi F, Cecchi P, Falcioni F, Marsciani A, Minak G, MURATORI G Arch Dis Child. 1998; 78(3):273-4.

PMID: 9613364 PMC: 1717498. DOI: 10.1136/adc.78.3.273.

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