Identification of a Gene (arpU) Controlling Muramidase-2 Export in Enterococcus Hirae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Oct 1

PMID 7592343

Citations 11

Authors

M M Lleo

R Fontana

M Solioz

Affiliations

Soon will be listed here.

Abstract

Muramidase-2 of Enterococcus hirae is a 74-kDa peptidoglycan hydrolase that plays a role in cell wall growth and division. To study its regulation, we isolated a mutant defective in muramidase-2 release under certain growth conditions. This mutant had cell walls which apparently lacked 74-kDa muramidase-2 but which accumulated two proteolytic fragments of 32 and 43 kDa, which exhibited muramidase-2 activity in the membrane fraction. By complementation cloning, we identified a 2.6-kb fragment of the E. hirae chromosome containing a gene cluster coding for proteins of 58 to 137 amino acids. One of these genes (arpU), which encoded a 15.9-kDa protein, was shown to complement the defect of the A9 mutant in trans. We propose that this gene may be involved in the regulation of muramidase-2 export.

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References

Holtje J, Tuomanen E . The murein hydrolases of Escherichia coli: properties, functions and impact on the course of infections in vivo. J Gen Microbiol. 1991; 137(3):441-54. DOI: 10.1099/00221287-137-3-441. View

Foster S, Johnstone K . Purification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM. Biochem J. 1987; 242(2):573-9. PMC: 1147743. DOI: 10.1042/bj2420573. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Terzaghi B, Sandine W . Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975; 29(6):807-13. PMC: 187084. DOI: 10.1128/am.29.6.807-813.1975. View

SHOCKMAN G, Kawamura T, Barrett J, Dolinger D . The autolytic peptidoglycan hydrolases of Streptococcus faecium. Ann Inst Pasteur Microbiol (1985). 1985; 136A(1):63-6. DOI: 10.1016/s0769-2609(85)80023-5. View

Solioz M, Waser M . Efficient electrotransformation of Enterococcus hirae with a new Enterococcus-Escherichia coli shuttle vector. Biochimie. 1990; 72(4):279-83. DOI: 10.1016/0300-9084(90)90084-t. View

Shungu D, Cornett J, SHOCKMAN G . Morphological and physiological study of autolytic-defective Streptococcus faecium strains. J Bacteriol. 1979; 138(2):598-608. PMC: 218217. DOI: 10.1128/jb.138.2.598-608.1979. View

Anderson D, McKay L . Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983; 46(3):549-52. PMC: 239314. DOI: 10.1128/aem.46.3.549-552.1983. View

Leclerc D, Asselin A . Detection of bacterial cell wall hydrolases after denaturing polyacrylamide gel electrophoresis. Can J Microbiol. 1989; 35(8):749-53. DOI: 10.1139/m89-125. View

10.

Barrett J, Dolinger D, Schramm V, SHOCKMAN G . The mechanism of soluble peptidoglycan hydrolysis by an autolytic muramidase. A processive exodisaccharidase. J Biol Chem. 1984; 259(19):11818-27. View

11.

Pugsley A . The complete general secretory pathway in gram-negative bacteria. Microbiol Rev. 1993; 57(1):50-108. PMC: 372901. DOI: 10.1128/mr.57.1.50-108.1993. View

12.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

13.

Chu C, Kariyama R, SHOCKMAN G . Cloning and sequence analysis of the muramidase-2 gene from Enterococcus hirae. J Bacteriol. 1992; 174(5):1619-25. PMC: 206558. DOI: 10.1128/jb.174.5.1619-1625.1992. View

14.

Joris B, Englebert S, Chu C, Kariyama R, SHOCKMAN G, Ghuysen J . Modular design of the Enterococcus hirae muramidase-2 and Streptococcus faecalis autolysin. FEMS Microbiol Lett. 1992; 70(3):257-64. DOI: 10.1016/0378-1097(92)90707-u. View

15.

Fontana R, Boaretti M, Grossato A, Tonin E, Lleo M, Satta G . Paradoxical response of Enterococcus faecalis to the bactericidal activity of penicillin is associated with reduced activity of one autolysin. Antimicrob Agents Chemother. 1990; 34(2):314-20. PMC: 171579. DOI: 10.1128/AAC.34.2.314. View

16.

SHOCKMAN G, Kolb J, TOENNIES G . Relations between bacterial cell wall synthesis, growth phase, and autolysis. J Biol Chem. 1958; 230(2):961-77. View

17.

Pooley H, SHOCKMAN G, Higgins M . Some properties of two autolytic-defective mutants of Streptococcus faecalis ATCC 9790. J Bacteriol. 1972; 109(1):423-31. PMC: 247294. DOI: 10.1128/jb.109.1.423-431.1972. View

18.

Mani N, Tobin P, Jayaswal R . Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis. J Bacteriol. 1993; 175(5):1493-9. PMC: 193237. DOI: 10.1128/jb.175.5.1493-1499.1993. View

19.

Dolinger D, SHOCKMAN G . The second peptidoglycan hydrolase of Streptococcus faecium ATCC 9790 covalently binds penicillin. J Bacteriol. 1989; 171(8):4355-61. PMC: 210212. DOI: 10.1128/jb.171.8.4355-4361.1989. View

20.

Kawamura T, SHOCKMAN G . Purification and some properties of the endogenous, autolytic N-acetylmuramoylhydrolase of Streptococcus faecium, a bacterial glycoenzyme. J Biol Chem. 1983; 258(15):9514-21. View