Identification of a Mutation in the Pst-phoU Operon That Reduces Pathogenicity of an Escherichia Coli Strain Causing Septicemia in Pigs

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1995 Dec 1

PMID 7591158

Citations 26

Authors

F Daigle

J M Fairbrother

J Harel

Affiliations

Soon will be listed here.

Abstract

We used transposon (TnphoA) mutagenesis to study the role of virulence factors of pathogenic Escherichia coli strains associated with septicemia in calves and piglets. We have produced an avirulent and serum-sensitive mutant of wild-type pathogenic strain 5131 O115:K"V165":F165 and have localized and identified the TnphoA insertion in the pstC gene of the pst-phoU operon. This operon encodes the PstSCAB transporter and PhoU protein that negatively regulate the phosphate (Pho) regulon. This mutation is pleiotropic and could have an effect on pathogenicity and on the production of the surface polysaccharides of strain 5131. The mutant demonstrated restored repressibility of alkaline phosphatase and regained the capacity to resist serum and to survive systemically for at least 5 days in experimentally inoculated pigs when complemented with plasmid pAN92, bearing the pst-phoU operon.

Citing Articles

Robic K, Munier E, Effantin G, Lachat J, Naquin D, Gueguen E Front Plant Sci. 2023; 14:1154110.

PMID: 37223796 PMC: 10202176. DOI: 10.3389/fpls.2023.1154110.

Novel Genes Required for the Fitness of in Human Saliva.

Zhu L, Charbonneau A, Waller A, Olsen R, Beres S, Musser J mSphere. 2017; 2(6).

PMID: 29104937 PMC: 5663985. DOI: 10.1128/mSphereDirect.00460-17.

Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential.

Marden J, McClure E, Beka L, Graf J Front Microbiol. 2016; 7:1569.

PMID: 27790190 PMC: 5061737. DOI: 10.3389/fmicb.2016.01569.

Interplay between genetic regulation of phosphate homeostasis and bacterial virulence.

Chekabab S, Harel J, Dozois C Virulence. 2014; 5(8):786-93.

PMID: 25483775 PMC: 4601331. DOI: 10.4161/viru.29307.

Identification and characterization of outer membrane vesicle-associated proteins in Salmonella enterica serovar Typhimurium.

Bai J, Kim S, Ryu S, Yoon H Infect Immun. 2014; 82(10):4001-10.

PMID: 24935973 PMC: 4187864. DOI: 10.1128/IAI.01416-13.

References

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Harel J, Jacques M, Fairbrother J, Bosse M, Forget C . Cloning of determinants encoding F165(2) fimbriae from porcine septicaemic Escherichia coli confirms their identity as F1C fimbriae. Microbiology (Reading). 1995; 141 ( Pt 1):221-8. DOI: 10.1099/00221287-141-1-221. View

Gibson F, Cox G, Downie J, Radik J . A mutation affecting a second component of the F0 portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. The uncC424 allele. Biochem J. 1977; 164(1):193-8. PMC: 1164774. DOI: 10.1042/bj1640193. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Taylor P, Kroll H . Killing of an encapsulated strain of Escherichia coli by human serum. Infect Immun. 1983; 39(1):122-31. PMC: 347913. DOI: 10.1128/iai.39.1.122-131.1983. View

Jans D, Fimmel A, Langman L, JAMES L, Downie J, Senior A . Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli. Biochem J. 1983; 211(3):717-26. PMC: 1154419. DOI: 10.1042/bj2110717. View

Surin B, Rosenberg H, Cox G . Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol. 1985; 161(1):189-98. PMC: 214855. DOI: 10.1128/jb.161.1.189-198.1985. View

ORSKOV I, ORSKOV F . Escherichia coli in extra-intestinal infections. J Hyg (Lond). 1985; 95(3):551-75. PMC: 2129573. DOI: 10.1017/s0022172400060678. View

10.

Kohara Y, Akiyama K, Isono K . The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987; 50(3):495-508. DOI: 10.1016/0092-8674(87)90503-4. View

11.

Fairbrother J, Broes A, Jacques M, Lariviere S . Pathogenicity of Escherichia coli O115:K"V165" strains isolated from pigs with diarrhea. Am J Vet Res. 1989; 50(7):1029-36. View

12.

Stock J, Ninfa A, Stock A . Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989; 53(4):450-90. PMC: 372749. DOI: 10.1128/mr.53.4.450-490.1989. View

13.

Rao N, TORRIANI A . Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol. 1990; 4(7):1083-90. DOI: 10.1111/j.1365-2958.1990.tb00682.x. View

14.

TORRIANI A . From cell membrane to nucleotides: the phosphate regulon in Escherichia coli. Bioessays. 1990; 12(8):371-6. DOI: 10.1002/bies.950120804. View

15.

Mekalanos J . Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol. 1992; 174(1):1-7. PMC: 205668. DOI: 10.1128/jb.174.1.1-7.1992. View

16.

Harel J, Forget C, Daigle F, Dubreuil D, Jacques M, Fairbrother J . Molecular cloning of a determinant coding for fimbrial antigen F165(1), a Prs-like fimbrial antigen from porcine septicaemic Escherichia coli. J Gen Microbiol. 1992; 138(7):1495-502. DOI: 10.1099/00221287-138-7-1495. View

17.

Webb D, Rosenberg H, Cox G . Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices. J Biol Chem. 1992; 267(34):24661-8. View

18.

Ngeleka M, Harel J, Jacques M, Fairbrother J . Characterization of a polysaccharide capsular antigen of septicemic Escherichia coli O115:K "V165" :F165 and evaluation of its role in pathogenicity. Infect Immun. 1992; 60(12):5048-56. PMC: 258276. DOI: 10.1128/iai.60.12.5048-5056.1992. View

19.

Muda M, Rao N, TORRIANI A . Role of PhoU in phosphate transport and alkaline phosphatase regulation. J Bacteriol. 1992; 174(24):8057-64. PMC: 207544. DOI: 10.1128/jb.174.24.8057-8064.1992. View

20.

Harel J, Forget C, Ngeleka M, Jacques M, Fairbrother J . Isolation and characterization of adhesin-defective TnphoA mutants of septicaemic porcine Escherichia coli of serotype O115:K-:F165. J Gen Microbiol. 1992; 138(11):2337-45. DOI: 10.1099/00221287-138-11-2337. View