Helicobacter Pylori Expresses a Complex Surface Carbohydrate, Lewis X

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1995 Dec 1

PMID 7591106

Citations 34

Authors

R Sherburne

D E Taylor

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies (MAbs) specific for Lewis X (Lex) reacted with whole cells of Helicobacter pylori NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206 in immunoelectron microscopy and enzyme-linked immunosorbent assay (ELISA) experiments. These MAbs have documented specificity to Lex, whereas MAbs for Lea and Leb were negative in both immunoelectron microscopy and ELISA. H. pylori coccoid forms also reacted with the MAbs, whereas the flagellum lacking the sheath showed no reactivity. The Lex structures were associated with membrane fractions in the ELISA experiments, and silver-stained sodium dodecyl sulfate-polyacrylamide gels confirmed the presence of lipopolysaccharides which reacted with the MAbs in immunoblots. Serum from an H. pylori-infected individual contained immunoglobulins which blocked the binding of the Lex MAbs, indicating that part of the host immune response to H. pylori is to the Lex structure. The ability of this gastric pathogen to mimic an oncofetal antigen (self) could explain the down regulation of anti-H. pylori T-cell response seen in H. pylori-infected individuals.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Tsai C, Frasch C . A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982; 119(1):115-9. DOI: 10.1016/0003-2697(82)90673-x. View

Hammar L, Mansson S, Rohr T, Chester M, Ginsburg V, Lundblad A . Lewis phenotype of erythrocytes and Leb-active glycolipid in serum of pregnant women. Vox Sang. 1981; 40(1):27-33. DOI: 10.1111/j.1423-0410.1981.tb00665.x. View

Kannagi R, Nudelman E, Levery S, Hakomori S . A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1. J Biol Chem. 1982; 257(24):14865-74. View

Batteiger B, Newhall 5th W, Jones R . The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods. 1982; 55(3):297-307. DOI: 10.1016/0022-1759(82)90089-8. View

Warren J, Marshall B . Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983; 1(8336):1273-5. View

Fukushima K, Hirota M, Terasaki P, Wakisaka A, Togashi H, Chia D . Characterization of sialosylated Lewisx as a new tumor-associated antigen. Cancer Res. 1984; 44(11):5279-85. View

Booth L, Holdstock G, MacBride H, Hawtin P, Gibson J, Ireland A . Clinical importance of Campylobacter pyloridis and associated serum IgG and IgA antibody responses in patients undergoing upper gastrointestinal endoscopy. J Clin Pathol. 1986; 39(2):215-9. PMC: 499681. DOI: 10.1136/jcp.39.2.215. View

Rathbone B, Wyatt J, Worsley B, Shires S, Trejdosiewicz L, Heatley R . Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut. 1986; 27(6):642-7. PMC: 1433340. DOI: 10.1136/gut.27.6.642. View

10.

Taylor D, Hargreaves J, Ng L, SHERBANIUK R, Jewell L . Isolation and characterization of Campylobacter pyloridis from gastric biopsies. Am J Clin Pathol. 1987; 87(1):49-54. DOI: 10.1093/ajcp/87.1.49. View

11.

Goodwin C, Blincow E, Peterson G, Sanderson C, Cheng W, Marshall B . Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. J Infect Dis. 1987; 155(3):488-94. DOI: 10.1093/infdis/155.3.488. View

12.

Slomiany B, Bilski J, Sarosiek J, MURTY V, Dworkin B, VanHorn K . Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity. Biochem Biophys Res Commun. 1987; 144(1):307-14. DOI: 10.1016/s0006-291x(87)80511-9. View

13.

Leunk R, Johnson P, David B, Kraft W, Morgan D . Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol. 1988; 26(2):93-9. DOI: 10.1099/00222615-26-2-93. View

14.

Mosmann T, Coffman R . Heterogeneity of cytokine secretion patterns and functions of helper T cells. Adv Immunol. 1989; 46:111-47. DOI: 10.1016/s0065-2776(08)60652-5. View

15.

Larsen E, Palabrica T, Sajer S, Gilbert G, Wagner D, Furie B . PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Cell. 1990; 63(3):467-74. DOI: 10.1016/0092-8674(90)90443-i. View

16.

PETERSON W . Helicobacter pylori and peptic ulcer disease. N Engl J Med. 1991; 324(15):1043-8. DOI: 10.1056/NEJM199104113241507. View

17.

Negrini R, Lisato L, Zanella I, Cavazzini L, Gullini S, Villanacci V . Helicobacter pylori infection induces antibodies cross-reacting with human gastric mucosa. Gastroenterology. 1991; 101(2):437-45. DOI: 10.1016/0016-5085(91)90023-e. View

18.

Parsonnet J, Friedman G, Vandersteen D, Chang Y, Vogelman J, ORENTREICH N . Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991; 325(16):1127-31. DOI: 10.1056/NEJM199110173251603. View

19.

Graham D . Helicobacter pylori: its epidemiology and its role in duodenal ulcer disease. J Gastroenterol Hepatol. 1991; 6(2):105-13. DOI: 10.1111/j.1440-1746.1991.tb01448.x. View

20.

Crabtree J, Shallcross T, Heatley R, Wyatt J . Mucosal tumour necrosis factor alpha and interleukin-6 in patients with Helicobacter pylori associated gastritis. Gut. 1991; 32(12):1473-7. PMC: 1379245. DOI: 10.1136/gut.32.12.1473. View