Giardicidal Activity of Lactoferrin and N-terminal Peptides

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1995 Nov 1

PMID 7591103

Citations 17

Authors

J M Turchany

S B Aley

F D Gillin

Affiliations

Soon will be listed here.

Abstract

Human and bovine lactoferrins and their derived N-terminal peptides were giardicidal in vitro. Fe3+, but not Fe2+, protected trophozoites from both native lactoferrin and peptides, although the latter lack iron-binding sites. Other divalent metal ions protected only against native lactoferrin. Log-phase cells were more resistant to killing than stationary-phase cells. These studies suggest that lactoferrin, especially in the form of the N-terminal peptides, may be an important nonimmune component of host mucosal defenses against Giardia lamblia.

Citing Articles

Antiparasitic activity of the iron-containing milk protein lactoferrin and its potential derivatives against human intestinal and blood parasites.

Anand N Front Parasitol. 2025; 2():1330398.

PMID: 39816822 PMC: 11731944. DOI: 10.3389/fpara.2023.1330398.

Lactoferrin and Lysozyme Inhibit the Proteolytic Activity and Cytopathic Effect of Enzymes.

Martinez-Castillo M, Ramirez-Rico G, Shibayama M, de la Garza M, Serrano-Luna J Pathogens. 2024; 13(1).

PMID: 38251351 PMC: 10819050. DOI: 10.3390/pathogens13010044.

Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against .

Sangkanu S, Paul A, Chuprom J, Mitsuwan W, Boonhok R, Pereira M Vaccines (Basel). 2023; 11(1).

PMID: 36679941 PMC: 9863896. DOI: 10.3390/vaccines11010096.

Activity of Apo-Lactoferrin on Pathogenic Protozoa.

Reyes-Lopez M, Ramirez-Rico G, Serrano-Luna J, de la Garza M Pharmaceutics. 2022; 14(8).

PMID: 36015327 PMC: 9414845. DOI: 10.3390/pharmaceutics14081702.

Diverse Mechanisms of Antimicrobial Activities of Lactoferrins, Lactoferricins, and Other Lactoferrin-Derived Peptides.

Gruden S, Poklar Ulrih N Int J Mol Sci. 2021; 22(20).

PMID: 34681923 PMC: 8541349. DOI: 10.3390/ijms222011264.

References

de Vet B, VAN GOOL J . Lactoferrin and iron absorption in the small intestine. Acta Med Scand. 1974; 196(5):393-402. DOI: 10.1111/j.0954-6820.1974.tb01030.x. View

Tomita M, Takase M, Bellamy W, Shimamura S . A review: the active peptide of lactoferrin. Acta Paediatr Jpn. 1994; 36(5):585-91. DOI: 10.1111/j.1442-200x.1994.tb03250.x. View

Arnold R, Cole M, McGhee J . A bactericidal effect for human lactoferrin. Science. 1977; 197(4300):263-5. DOI: 10.1126/science.327545. View

Mason D, Taylor C . Distribution of transferrin, ferritin, and lactoferrin in human tissues. J Clin Pathol. 1978; 31(4):316-27. PMC: 1145269. DOI: 10.1136/jcp.31.4.316. View

Arnold R, Brewer M, Gauthier J . Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms. Infect Immun. 1980; 28(3):893-8. PMC: 551035. DOI: 10.1128/iai.28.3.893-898.1980. View

Brock J . Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant. Arch Dis Child. 1980; 55(6):417-21. PMC: 1626933. DOI: 10.1136/adc.55.6.417. View

Arnold R, Russell J, CHAMPION W, Gauthier J . Bactericidal activity of human lactoferrin: influence of physical conditions and metabolic state of the target microorganism. Infect Immun. 1981; 32(2):655-60. PMC: 351497. DOI: 10.1128/iai.32.2.655-660.1981. View

Reiter B . The biological significance of lactoferrin. Int J Tissue React. 1983; 5(1):87-96. View

Vaara M, Vaara T . Polycations as outer membrane-disorganizing agents. Antimicrob Agents Chemother. 1983; 24(1):114-22. PMC: 185113. DOI: 10.1128/AAC.24.1.114. View

10.

Peterson K, Alderete J . Iron uptake and increased intracellular enzyme activity follow host lactoferrin binding by Trichomonas vaginalis receptors. J Exp Med. 1984; 160(2):398-410. PMC: 2187462. DOI: 10.1084/jem.160.2.398. View

11.

Rohrer L, Winterhalter K, Eckert J, Kohler P . Killing of Giardia lamblia by human milk is mediated by unsaturated fatty acids. Antimicrob Agents Chemother. 1986; 30(2):254-7. PMC: 180529. DOI: 10.1128/AAC.30.2.254. View

12.

Lehrer R, Ganz T, Selsted M . Defensins: endogenous antibiotic peptides of animal cells. Cell. 1991; 64(2):229-30. DOI: 10.1016/0092-8674(91)90632-9. View

13.

Schroder G, Brandenburg K, Seydel U . Polymyxin B induces transient permeability fluctuations in asymmetric planar lipopolysaccharide/phospholipid bilayers. Biochemistry. 1992; 31(3):631-8. DOI: 10.1021/bi00118a001. View

14.

Sanchez L, Calvo M, Brock J . Biological role of lactoferrin. Arch Dis Child. 1992; 67(5):657-61. PMC: 1793702. DOI: 10.1136/adc.67.5.657. View

15.

Bellamy W, Takase M, Yamauchi K, Wakabayashi H, Kawase K, Tomita M . Identification of the bactericidal domain of lactoferrin. Biochim Biophys Acta. 1992; 1121(1-2):130-6. DOI: 10.1016/0167-4838(92)90346-f. View

16.

Eisenhauer P, Harwig S, Lehrer R . Cryptdins: antimicrobial defensins of the murine small intestine. Infect Immun. 1992; 60(9):3556-65. PMC: 257361. DOI: 10.1128/iai.60.9.3556-3565.1992. View

17.

Yamauchi K, Tomita M, Giehl T, Ellison 3rd R . Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment. Infect Immun. 1993; 61(2):719-28. PMC: 302785. DOI: 10.1128/iai.61.2.719-728.1993. View

18.

Bellamy W, Takase M, Wakabayashi H, Kawase K, Tomita M . Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J Appl Bacteriol. 1992; 73(6):472-9. DOI: 10.1111/j.1365-2672.1992.tb05007.x. View

19.

Bellamy W, Wakabayashi H, Takase M, Kawase K, Shimamura S, Tomita M . Killing of Candida albicans by lactoferricin B, a potent antimicrobial peptide derived from the N-terminal region of bovine lactoferrin. Med Microbiol Immunol. 1993; 182(2):97-105. DOI: 10.1007/BF00189377. View

20.

Bellamy W, Wakabayashi H, Takase M, Kawase K, Shimamura S, Tomita M . Role of cell-binding in the antibacterial mechanism of lactoferricin B. J Appl Bacteriol. 1993; 75(5):478-84. View