Intergeneric Transfer and Functional Expression of the Tomato Disease Resistance Gene Pto

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1995 Oct 1

PMID 7580250

Citations 28

Authors

C M Rommens

J M Salmeron

G E Oldroyd

B J Staskawicz

Affiliations

Soon will be listed here.

Abstract

Plant disease resistance loci have been used successfully in breeding programs to transfer traits from resistant germplasm to susceptible plant cultivars. The molecular cloning of plant disease resistance genes now permits the transfer of such traits across species boundaries by genetic transformation of recipient hosts. The tomato disease resistance gene Pto confers resistance to strains of the bacterial pathogen Pseudomonas syringae pv tomato expressing the avirulence gene avrPto. Transformation of Nicotiana benthamiana with Pto results in specific resistance to P. s. pv tabaci strains carrying avrPto. The resistant phenotype is manifested by a strong inhibition of bacterial growth and the ability to exhibit a hypersensitive response. Resistance cosegregates with the Pto gene in transgene selfings and testcrosses. Our results demonstrate the conservation of disease resistance functions across genus boundaries and suggest that the utility of host-specific resistance genes can be extended by intergeneric transfer.

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References

Mindrinos M, Katagiri F, Yu G, Ausubel F . The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994; 78(6):1089-99. DOI: 10.1016/0092-8674(94)90282-8. View

Whalen M, Stall R, Staskawicz B . Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. Proc Natl Acad Sci U S A. 1988; 85(18):6743-7. PMC: 282054. DOI: 10.1073/pnas.85.18.6743. View

Martin G, Frary A, Wu T, Brommonschenkel S, Chunwongse J, Earle E . A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death. Plant Cell. 1994; 6(11):1543-52. PMC: 160542. DOI: 10.1105/tpc.6.11.1543. View

Kearney B, Staskawicz B . Widespread distribution and fitness contribution of Xanthomonas campestris avirulence gene avrBs2. Nature. 1990; 346(6282):385-6. DOI: 10.1038/346385a0. View

Rommens C, Salmeron J, Baulcombe D, Staskawicz B . Use of a gene expression system based on potato virus X to rapidly identify and characterize a tomato Pto homolog that controls fenthion sensitivity. Plant Cell. 1995; 7(3):249-57. PMC: 160779. DOI: 10.1105/tpc.7.3.249. View

Jones J, Shlumukov L, Carland F, English J, Scofield S, Bishop G . Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Res. 1992; 1(6):285-97. DOI: 10.1007/BF02525170. View

Kunkel B, Bent A, Dahlbeck D, Innes R, Staskawicz B . RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2. Plant Cell. 1993; 5(8):865-75. PMC: 160322. DOI: 10.1105/tpc.5.8.865. View

Klimyuk V, Carroll B, Thomas C, Jones J . Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J. 1993; 3(3):493-4. DOI: 10.1111/j.1365-313x.1993.tb00169.x. View

Loh Y, Martin G . The Pto bacterial resistance gene and the Fen insecticide sensitivity gene encode functional protein kinases with serine/threonine specificity. Plant Physiol. 1995; 108(4):1735-9. PMC: 157555. DOI: 10.1104/pp.108.4.1735. View

10.

Ellis J, Lawrence G, Finnegan E, Anderson P . Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci U S A. 1995; 92(10):4185-8. PMC: 41908. DOI: 10.1073/pnas.92.10.4185. View

11.

Jones D, Thomas C, Hammond-Kosack K, Balint-Kurti P, Jones J . Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science. 1994; 266(5186):789-93. DOI: 10.1126/science.7973631. View

12.

Martin G, Brommonschenkel S, Chunwongse J, Frary A, Ganal M, Spivey R . Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science. 1993; 262(5138):1432-6. DOI: 10.1126/science.7902614. View

13.

Figurski D, Helinski D . Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979; 76(4):1648-52. PMC: 383447. DOI: 10.1073/pnas.76.4.1648. View

14.

Carland F, Staskawicz B . Genetic characterization of the Pto locus of tomato: semi-dominance and cosegregation of resistance to Pseudomonas syringae pathovar tomato and sensitivity to the insecticide Fenthion. Mol Gen Genet. 1993; 239(1-2):17-27. DOI: 10.1007/BF00281596. View

15.

Salmeron J, Barker S, Carland F, Mehta A, Staskawicz B . Tomato mutants altered in bacterial disease resistance provide evidence for a new locus controlling pathogen recognition. Plant Cell. 1994; 6(4):511-20. PMC: 160454. DOI: 10.1105/tpc.6.4.511. View

16.

Bent A, Kunkel B, Dahlbeck D, Brown K, Schmidt R, Giraudat J . RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science. 1994; 265(5180):1856-60. DOI: 10.1126/science.8091210. View

17.

Salmeron J, Staskawicz B . Molecular characterization and hrp dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato [corrected]. Mol Gen Genet. 1993; 239(1-2):6-16. DOI: 10.1007/BF00281595. View