Cloning and Complete Sequence of the DNA Polymerase-encoding Gene (BstpolI) and Characterisation of the Klenow-like Fragment from Bacillus Stearothermophilus

Overview

Journal Gene

Specialty Molecular Biology

Date 1995 Sep 22

PMID 7557480

Citations 11

Authors

S M Phang

C Y Teo

E Lo

V W Wong

Affiliations

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Abstract

A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.

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