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Prospective Comparison of Direct Immunofluorescence and Conventional Staining Methods for Detection of Giardia and Cryptosporidium Spp. in Human Fecal Specimens

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1995 Jun 1
PMID 7544365
Citations 26
Authors
A J Alles
M A Waldron
L S Sierra
A R Mattia
Affiliations
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Abstract

In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).

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