Effects of Spo0 Mutations on Spo0A Promoter Switching at the Initiation of Sporulation in Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Aug 1

PMID 7543482

Citations 12

Authors

T Chibazakura

F Kawamura

K Asai

H Takahashi

Affiliations

Soon will be listed here.

Abstract

Transcriptional analyses of the Bacillus subtilis sporulation initiator gene spo0A revealed that promoter switching from the vegetative (Pv) to the sporulation-specific (Ps) promoter did not occur in the spo0A, spo0B, spo0E, spo0F, and spo0H mutants. The sof-1 mutation in spo0A restored the promoter switching in the spo0F mutant. These results strongly suggest that Spo0A plays a central role in the regulation of its own promoter switching.

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References

Strauch M, Webb V, Spiegelman G, Hoch J . The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci U S A. 1990; 87(5):1801-5. PMC: 53571. DOI: 10.1073/pnas.87.5.1801. View

Yamashita S, Kawamura F, Yoshikawa H, Takahashi H, Kobayashi Y, Saito H . Dissection of the expression signals of the spoA gene of Bacillus subtilis: glucose represses sporulation-specific expression. J Gen Microbiol. 1989; 135(5):1335-45. DOI: 10.1099/00221287-135-5-1335. View

Burbulys D, Trach K, Hoch J . Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 1991; 64(3):545-52. DOI: 10.1016/0092-8674(91)90238-t. View

Perego M, Hoch J . Negative regulation of Bacillus subtilis sporulation by the spo0E gene product. J Bacteriol. 1991; 173(8):2514-20. PMC: 207815. DOI: 10.1128/jb.173.8.2514-2520.1991. View

Chibazakura T, Kawamura F, Takahashi H . Differential regulation of spo0A transcription in Bacillus subtilis: glucose represses promoter switching at the initiation of sporulation. J Bacteriol. 1991; 173(8):2625-32. PMC: 207829. DOI: 10.1128/jb.173.8.2625-2632.1991. View

Satola S, Kirchman P, Moran Jr C . Spo0A binds to a promoter used by sigma A RNA polymerase during sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1991; 88(10):4533-7. PMC: 51695. DOI: 10.1073/pnas.88.10.4533. View

Perego M, Wu J, Spiegelman G, Hoch J . Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis. Gene. 1991; 100:207-12. DOI: 10.1016/0378-1119(91)90368-l. View

Trach K, Burbulys D, Strauch M, Wu J, Dhillon N, Jonas R . Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay. Res Microbiol. 1991; 142(7-8):815-23. DOI: 10.1016/0923-2508(91)90060-n. View

Predich M, Nair G, Smith I . Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H. J Bacteriol. 1992; 174(9):2771-8. PMC: 205927. DOI: 10.1128/jb.174.9.2771-2778.1992. View

10.

Strauch M, Trach K, Day J, Hoch J . Spo0A activates and represses its own synthesis by binding at its dual promoters. Biochimie. 1992; 74(7-8):619-26. DOI: 10.1016/0300-9084(92)90133-y. View

11.

Strauch M, Wu J, Jonas R, Hoch J . A positive feedback loop controls transcription of the spoOF gene, a component of the sporulation phosphorelay in Bacillus subtilis. Mol Microbiol. 1993; 7(6):967-74. DOI: 10.1111/j.1365-2958.1993.tb01188.x. View

12.

Ohlsen K, Grimsley J, Hoch J . Deactivation of the sporulation transcription factor Spo0A by the Spo0E protein phosphatase. Proc Natl Acad Sci U S A. 1994; 91(5):1756-60. PMC: 43242. DOI: 10.1073/pnas.91.5.1756. View

13.

Leighton T, Doi R . The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem. 1971; 246(10):3189-95. View

14.

Hoch J . Genetics of bacterial sporulation. Adv Genet. 1976; 18:69-98. DOI: 10.1016/s0065-2660(08)60437-x. View

15.

Piggot P, Coote J . Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976; 40(4):908-62. PMC: 413989. DOI: 10.1128/br.40.4.908-962.1976. View

16.

Kawamura F, Saito H . Isolation and mapping of a new suppressor mutation of an early sporulation gene spoOF mutation in Bacillus subtilis. Mol Gen Genet. 1983; 192(3):330-4. DOI: 10.1007/BF00392171. View

17.

Hoch J, Trach K, Kawamura F, Saito H . Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein. J Bacteriol. 1985; 161(2):552-5. PMC: 214917. DOI: 10.1128/jb.161.2.552-555.1985. View

18.

FERRARI F, Trach K, Lecoq D, Spence J, Ferrari E, Hoch J . Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985; 82(9):2647-51. PMC: 397621. DOI: 10.1073/pnas.82.9.2647. View

19.

Kudoh J, Ikeuchi T, Kurahashi K . Nucleotide sequences of the sporulation gene spo0A and its mutant genes of Bacillus subtilis. Proc Natl Acad Sci U S A. 1985; 82(9):2665-8. PMC: 397625. DOI: 10.1073/pnas.82.9.2665. View

20.

Yamashita S, Yoshikawa H, Kawamura F, Takahashi H, Yamamoto T, Kobayashi Y . The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions. Mol Gen Genet. 1986; 205(1):28-33. DOI: 10.1007/BF02428029. View