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Ex Vivo Expansion of Cord Blood-derived Stem Cells and Progenitors

Overview
Journal Blood Cells
Specialty Hematology
Date 1994 Jan 1
PMID 7538352
Citations 11
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Abstract

Cord blood-derived CD34+ cells have proved superior to adult marrow or elicited peripheral blood in ex vivo cell and progenitor expansion. In addition, cord blood-derived cells with a phenotype and function of early stem cells (e.g., long-term culture-initiating cells) can be expanded in vitro in the presence of a combination of cytokines by 15- to 20-fold over 7-14 days. These observations suggest that ex vivo expansion of cord blood CD34+ cells will permit improved engraftment of adults and will prove effective in retroviral-mediated gene transfection of early stem cell populations.

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