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"Pseudoclusters" and Typing by Random Amplification of Polymorphic DNA of Aspergillus Fumigatus

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1995 Feb 1
PMID 7538151
Citations 7
Authors
K W Loudon
A P Coke
J P Burnie
Affiliations
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Abstract

This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus.

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References
1.
Gerson S, Talbot G, Hurwitz S, Strom B, Lusk E, Cassileth P . Prolonged granulocytopenia: the major risk factor for invasive pulmonary aspergillosis in patients with acute leukemia. Ann Intern Med. 1984; 100(3):345-51. DOI: 10.7326/0003-4819-100-3-345. View
2.
Denning D, Stevens D . Antifungal and surgical treatment of invasive aspergillosis: review of 2,121 published cases. Rev Infect Dis. 1990; 12(6):1147-201. DOI: 10.1093/clinids/12.6.1147. View
3.
Cohen J, Holden D . Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J Clin Microbiol. 1992; 30(11):2991-3. PMC: 270566. DOI: 10.1128/jcm.30.11.2991-2993.1992. View
4.
Loudon K, Coke A, Burnie J, Lucas G, Liu Yin J . Invasive aspergillosis: clusters and sources?. J Med Vet Mycol. 1994; 32(3):217-24. DOI: 10.1080/02681219480000281. View
5.
Girardin H, Latge J, Srikantha T, Morrow B, Soll D . Development of DNA probes for fingerprinting Aspergillus fumigatus. J Clin Microbiol. 1993; 31(6):1547-54. PMC: 265576. DOI: 10.1128/jcm.31.6.1547-1554.1993. View