Carbohydrate Antigens of Human Megakaryocytes and Platelet Glycoproteins: a Comparative Study

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1994 Sep 1

PMID 7532635

Citations 5

Authors

S E Baldus

J Thiele

A Charles

F G Hanisch

R Fischer

Affiliations

Soon will be listed here.

Abstract

Until now, carbohydrate antigens of human megakaryocytes have not been studied very extensively. For this reason, we investigated the staining pattern of 25 lectins and carbohydrate-specific monoclonal antibodies on paraffin-embedded trephine biopsies and acetone-fixed smears from patients with reactive and neoplastic bone marrow lesions. A biotin-streptavidin-alkaline phosphatase assay was used to visualize the binding of lectins or antibodies. Ulex europaeus agglutinin I (UEA-I) stained megakaryocytes in all cases tested. Monoclonal antibodies detecting fucosylated Lewis type 2 chain antigens (19-OLE, 12-4LE and LeuM1) were also reactive. Several lectins detecting backbone and core oligosaccharides [Helix pomatia agglutinin (HPA), peanut agglutinin (PNA), Erythrina cristagalli agglutinin (ECA), soybean agglutinin (SBA)] bound to megakaryocytes only after neuraminidase digestion. Moreover, we investigated human platelet lysates to gain some information about the carbohydrate residues of platelet glycoproteins which are synthesized by megakaryocytes. The carbohydrate expression of platelets showed striking similarities to that of megakaryocytes. Immunoblotting experiments revealed a strong binding of UEA-I, 19-OLE and 12-4LE to a band isographic to glycoprotein (gp) Ib. After desialylation of glycoproteins transblotted to nitrocellulose, ECA and PNA also reacted with a band of this molecular weight. Gp Ib is known to contain a mucin-like peptide core with a great number of potential O-glycosylation sites. Therefore, it is tempting to speculate that carbohydrate residues characterized in this study are involved in the complex biological interactions of gp Ib.

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References

Clemetson K, Naim H, LUSCHER E . Relationship between glycocalicin and glycoprotein Ib of human platelets. Proc Natl Acad Sci U S A. 1981; 78(5):2712-6. PMC: 319427. DOI: 10.1073/pnas.78.5.2712. View

Tsuji T, Osawa T . Structures of the carbohydrate chains of membrane glycoproteins IIb and IIIa of human platelets. J Biochem. 1986; 100(5):1387-98. DOI: 10.1093/oxfordjournals.jbchem.a121845. View

Hanjan S, Kearney J, Cooper M . A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982; 23(2):172-88. DOI: 10.1016/0090-1229(82)90106-4. View

Hoffman R . Regulation of megakaryocytopoiesis. Blood. 1989; 74(4):1196-212. View

Korrel S, Clemetson K, van Halbeek H, Kamerling J, Sixma J, Vliegenthart J . Identification of a tetrasialylated monofucosylated tetraantennary N-linked carbohydrate chain in human platelet glycocalicin. FEBS Lett. 1988; 228(2):321-6. DOI: 10.1016/0014-5793(88)80024-3. View

Abgrall J, Renard I, Berthou C, Sensebe L, Guern G, Autrand C . Carbohydrate composition of human megakaryocyte membranes in culture: identification using binding of seven lectins. Int J Cell Cloning. 1991; 9(3):233-8. DOI: 10.1002/stem.5530090307. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Feizi T, Gooi H, Childs R, Picard J, Uemura K, Loomes L . Tumour-associated and differentiation antigens on the carbohydrate moieties of mucin-type glycoproteins. Biochem Soc Trans. 1984; 12(4):591-6. DOI: 10.1042/bst0120591. View

Schumacher U, Horny H, Welsch U, Kaiserling E . Lectin histochemistry of human bone marrow: investigation of trephine biopsy specimens in normal and reactive states and neoplastic disorders. Histochem J. 1991; 23(5):215-20. DOI: 10.1007/BF01462243. View

10.

Tsuji T, Tsunehisa S, Watanabe Y, Yamamoto K, Tohyama H, Osawa T . The carbohydrate moiety of human platelet glycocalicin. J Biol Chem. 1983; 258(10):6335-9. View

11.

Kaladas P, KABAT E, Iglesias J, Lis H, Sharon N . Immunochemical studies on the combining site of the D-galactose/N-acetyl-D-galactosamine specific lectin from Erythrina cristagalli seeds. Arch Biochem Biophys. 1982; 217(2):624-37. DOI: 10.1016/0003-9861(82)90544-6. View

12.

Lopez J, Ludwig E, McCarthy B . Polymorphism of human glycoprotein Ib alpha results from a variable number of tandem repeats of a 13-amino acid sequence in the mucin-like macroglycopeptide region. Structure/function implications. J Biol Chem. 1992; 267(14):10055-61. View

13.

Irimura T, Nicolson G . Carbohydrate chain analysis by lectin binding to electrophoretically separated glycoproteins from murine B16 melanoma sublines of various metastatic properties. Cancer Res. 1984; 44(2):791-8. View

14.

Vierbuchen M, Uhlenbruck G, Ortmann M, Dufhues G, Fischer R . Occurrence and distribution of glycoconjugates in human tissues as detected by the Erythrina cristagalli lectin. J Histochem Cytochem. 1988; 36(4):367-76. DOI: 10.1177/36.4.3346539. View

15.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

16.

Thomas D, WINZLER R . Structural studies on human erythrocyte glycoproteins. Alkali-labile oligosaccharides. J Biol Chem. 1969; 244(21):5943-6. View

17.

Cordell J, Falini B, Erber W, Ghosh A, Abdulaziz Z, MacDonald S . Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984; 32(2):219-29. DOI: 10.1177/32.2.6198355. View

18.

McDONALD T . The regulation of megakaryocyte and platelet production. Int J Cell Cloning. 1989; 7(3):139-55. DOI: 10.1002/stem.5530070302. View

19.

Lotan R, Skutelsky E, Danon D, Sharon N . The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem. 1975; 250(21):8518-23. View

20.

van der Valk P, Mullink H, Huijgens P, Tadema T, Vos W, Meijer C . Immunohistochemistry in bone marrow diagnosis. Value of a panel of monoclonal antibodies on routinely processed bone marrow biopsies. Am J Surg Pathol. 1989; 13(2):97-106. View