Structural Studies of the Side Chain of Outer Membrane Lipopolysaccharide from Pseudomonas Syringae Pv. Coriandricola W-43

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1994 Nov 1

PMID 7525536

Citations 1

Authors

S Das

M Ramm

H Kochanowski

S Basu

Affiliations

Soon will be listed here.

Abstract

The lipopolysaccharide (LPS) was isolated from Pseudomonas syringae pv. coriandricola W-43 by hot phenol-water extraction. Rhamnose and 3-N-acetyl-3-deoxyfucose were found to be the major sugar constituents of the LPS together with N-acetylglucosamine, N-acetylgalactosamine, heptose, and 3-deoxy-D-manno-octulosonic acid (Kdo). The main fatty acids of lipid A of the LPS were 3-OH-C:10, C12:0, 2-OH-C12:0, and 3-OH-C12:0. The O-specific polysaccharide liberated from the LPS by mild-acid hydrolysis was purified by gel permeation chromatography. The compositional analysis of the O-specific polysaccharide revealed the presence of L-rhamnose and 3-N-acetyl-3-deoxy-D-fucose in a molar ratio of 4:1. The primary structure of the O-specific polysaccharide was established by methylation analysis together with 1H and 13C nuclear magnetic resonance spectroscopy, including two-dimensional shift-correlated and one-dimensional nuclear Overhauser effect spectroscopy. The polysaccharide moiety was found to consist of a tetrasaccharide rhamnan backbone, and 3-N-acetyl-3-deoxy-D-fucose constitutes the side chain of the branched pentasaccharide repeating unit of the polysaccharide.

Citing Articles

Immunochemical characterization of O polysaccharides composing the alpha-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies.

Ovod V, Rudolph K, Knirel Y, Krohn K J Bacteriol. 1996; 178(22):6459-65.

PMID: 8932301 PMC: 178531. DOI: 10.1128/jb.178.22.6459-6465.1996.

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