An Immunohistochemical Study of Tissue Transglutaminase in Gliomas with Reference to Their Cell Dying Processes

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1994 Oct 1

PMID 7524329

Citations 6

Authors

T Iwaki

M Miyazono

T Hitosumatsu

J Tateishi

Affiliations

Soon will be listed here.

Abstract

Tissue transglutaminase is a Ca(2+)-dependent enzyme that catalyzes the formation of protein cross-links by an acyl transfer reaction. Recent reports have suggested that tissue transglutaminase is induced by tumor progression and apoptosis. In this study we immunohistochemically investigated a series of gliomas by using an antiserum against a dodecapeptide from the COOH-terminal of tissue transglutaminase. Among the gliomas the presence of positive immunoreactivity tended to increase in malignant counterparts. It is also noteworthy to mention that glioblastoma cells surrounding the zonal necrosis in a palisade fashion were strongly immunolabeled. The degenerating products in tumor cells, such as round granulated bodies, were primarily immunopositive, whereas Rosenthal fibers were negative. Dying cells through apoptosis in the metastatic brain tumors could be easily recognized by the presence of tissue transglutaminase. In conclusion, tissue transglutaminase may therefore be valuable in the prognostic characterization of gliomas with respect to the detection of dying cells. However, the appearance of tissue transglutaminase-positive neoplastic cells was not limited to apoptotic bodies but could also be detected in necrobiotic cell nests.

Citing Articles

Biological Implications and Functional Significance of Transglutaminase Type 2 in Nervous System Tumors.

Buccarelli M, Castellani G, Fiorentino V, Pizzimenti C, Beninati S, Ricci-Vitiani L Cells. 2024; 13(8.

PMID: 38667282 PMC: 11048792. DOI: 10.3390/cells13080667.

Effects of endotoxin on expression of ras, p53 and bcl-2 oncoprotein in hepatocarcinogenesis induced by thioacetamide in rats.

Yang J, Han D, Liang Q, Zhao J, Hao S, Ma X World J Gastroenterol. 2016; 3(4):213-7.

PMID: 27053867 PMC: 4806235. DOI: 10.3748/wjg.v3.i4.213.

Apoptotic markers for primary brain tumor prognosis.

Konstantinidou A, Korkolopoulou P, Patsouris E J Neurooncol. 2005; 72(2):151-6.

PMID: 15925995 DOI: 10.1007/s11060-004-3345-z.

Apoptosis detected with monoclonal antibody to single-stranded DNA is a predictor of recurrence in intracranial meningiomas.

Konstantinidou A, Korkolopoulou P, Patsouris E, Mahera H, Hranioti S, Kotsiakis X J Neurooncol. 2002; 55(1):1-9.

PMID: 11804277 DOI: 10.1023/a:1012941202510.

Distinct radiochemotherapy protocols differentially influence cellular proliferation and expression of p53 and Bcl-2 in glioblastoma multiforme relapses in vivo.

Deininger M, Grote E, Wickboldt J, Meyermann R J Neurooncol. 2000; 48(2):121-9.

PMID: 11083075 DOI: 10.1023/a:1006462618800.

References

Green H . Terminal differentiation of cultured human epidermal cells. Cell. 1977; 11(2):405-16. DOI: 10.1016/0092-8674(77)90058-7. View

Carson D, Ribeiro J . Apoptosis and disease. Lancet. 1993; 341(8855):1251-4. DOI: 10.1016/0140-6736(93)91154-e. View

Fukui M, Iwaki T, Sawa H, Inoue T, Takeshita I, Kitamura K . Proliferative activity of meningiomas as evaluated by bromodeoxyuridine uptake examination. Acta Neurochir (Wien). 1986; 81(3-4):135-41. DOI: 10.1007/BF01401236. View

Iwaki T, Takeshita I, Fukui M, Kitamura K . Cell kinetics of the malignant evolution of meningothelial meningioma. Acta Neuropathol. 1987; 74(3):243-7. DOI: 10.1007/BF00688188. View

Fesus L, Thomazy V, Falus A . Induction and activation of tissue transglutaminase during programmed cell death. FEBS Lett. 1987; 224(1):104-8. DOI: 10.1016/0014-5793(87)80430-1. View

Ikura K, Nasu T, Yokota H, Tsuchiya Y, Sasaki R, Chiba H . Amino acid sequence of guinea pig liver transglutaminase from its cDNA sequence. Biochemistry. 1988; 27(8):2898-905. DOI: 10.1021/bi00408a035. View

Fesus L, Thomazy V, AUTUORI F, Ceru M, Tarcsa E, Piacentini M . Apoptotic hepatocytes become insoluble in detergents and chaotropic agents as a result of transglutaminase action. FEBS Lett. 1989; 245(1-2):150-4. DOI: 10.1016/0014-5793(89)80210-8. View

Iwaki T, Liem R, Goldman J . Alpha B-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain. Cell. 1989; 57(1):71-8. DOI: 10.1016/0092-8674(89)90173-6. View

Thomazy V, Fesus L . Differential expression of tissue transglutaminase in human cells. An immunohistochemical study. Cell Tissue Res. 1989; 255(1):215-24. DOI: 10.1007/BF00229084. View

10.

Posnett D, Tam J . Multiple antigenic peptide method for producing antipeptide site-specific antibodies. Methods Enzymol. 1989; 178:739-46. DOI: 10.1016/0076-6879(89)78048-4. View

11.

Arends M, Morris R, Wyllie A . Apoptosis. The role of the endonuclease. Am J Pathol. 1990; 136(3):593-608. PMC: 1877493. View

12.

Ichinose A, Bottenus R, Davie E . Structure of transglutaminases. J Biol Chem. 1990; 265(23):13411-4. View

13.

Gentile V, Saydak M, Chiocca E, Akande O, Birckbichler P, Lee K . Isolation and characterization of cDNA clones to mouse macrophage and human endothelial cell tissue transglutaminases. J Biol Chem. 1991; 266(1):478-83. View

14.

KNIGHT C, Rees R, Griffin M . Apoptosis: a potential role for cytosolic transglutaminase and its importance in tumour progression. Biochim Biophys Acta. 1991; 1096(4):312-8. DOI: 10.1016/0925-4439(91)90067-j. View

15.

Greenberg C, Birckbichler P, Rice R . Transglutaminases: multifunctional cross-linking enzymes that stabilize tissues. FASEB J. 1991; 5(15):3071-7. DOI: 10.1096/fasebj.5.15.1683845. View

16.

Iwaki T, Tateishi J . Immunohistochemical demonstration of alphaB-crystallin in hamartomas of tuberous sclerosis. Am J Pathol. 1991; 139(6):1303-8. PMC: 1886473. View

17.

Yamanishi K, Inazawa J, Liew F, Nonomura K, Ariyama T, Yasuno H . Structure of the gene for human transglutaminase 1. J Biol Chem. 1992; 267(25):17858-63. View

18.

Gavrieli Y, Sherman Y, Ben-Sasson S . Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol. 1992; 119(3):493-501. PMC: 2289665. DOI: 10.1083/jcb.119.3.493. View

19.

Shweiki D, Itin A, Soffer D, Keshet E . Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature. 1992; 359(6398):843-5. DOI: 10.1038/359843a0. View

20.

Plate K, Breier G, Weich H, Risau W . Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo. Nature. 1992; 359(6398):845-8. DOI: 10.1038/359845a0. View