Artificial Mosaic Protein Containing Antigenic Epitopes of Hepatitis E Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1994 Nov 1

PMID 7523696

Citations 5

Authors

Y E Khudyakov

M O Favorov

N S Khudyakova

M E Cong

B P Holloway

N Padhye

S B Lambert

D L Jue

H A Fields

Affiliations

Soon will be listed here.

Abstract

A synthetic gene encoding an artificial polypeptide composed of antigenic epitopes of the hepatitis E virus (HEV) proteins was constructed from short oligodeoxyribonucleotides by using PCR. The polypeptide comprises a mosaic of three antigenically active dominant regions from the protein encoded by open reading frame 2 (ORF2), one antigenically active region from the protein encoded by ORF3 of the Burmese HEV strain, and one antigenically active region from the protein encoded by ORF3 of the Mexican HEV strain. The mosaic protein was expressed in Escherichia coli as a chimera with glutathione S-transferase or beta-galactosidase. Guinea pig sera containing antibodies to the corresponding HEV synthetic peptides were used to demonstrate by Western immunoblot analysis and enzyme immunoassay the presence and accessibility of all HEV-specific antigenic epitopes introduced into the mosaic protein. Both the glutathione S-transferase and beta-galactosidase hybrid proteins were analyzed by using a panel of human anti-HEV-positive and -negative sera. The data obtained strongly indicate a diagnostic potential for the mosaic protein.

Citing Articles

Current Knowledge on Hepatitis E.

Perez-Gracia M, Garcia M, Suay B, Mateos-Lindemann M J Clin Transl Hepatol. 2015; 3(2):117-26.

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Diagnosis of hepatitis E.

Aggarwal R Nat Rev Gastroenterol Hepatol. 2012; 10(1):24-33.

PMID: 23026902 DOI: 10.1038/nrgastro.2012.187.

Seroprevalence study in forestry workers from eastern Germany using novel genotype 3- and rat hepatitis E virus-specific immunoglobulin G ELISAs.

Dremsek P, Wenzel J, Johne R, Ziller M, Hofmann J, Groschup M Med Microbiol Immunol. 2011; 201(2):189-200.

PMID: 22179131 DOI: 10.1007/s00430-011-0221-2.

Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal antibodies.

Riddell M, Li F, Anderson D J Virol. 2000; 74(17):8011-7.

PMID: 10933710 PMC: 112333. DOI: 10.1128/jvi.74.17.8011-8017.2000.

Antigenic domains of the open reading frame 2-encoded protein of hepatitis E virus.

Khudyakov Y, Lopareva E, Jue D, Crews T, Thyagarajan S, Fields H J Clin Microbiol. 1999; 37(9):2863-71.

PMID: 10449466 PMC: 85398. DOI: 10.1128/JCM.37.9.2863-2871.1999.

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