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Modified Technique for Efficient Detection of Microsporidia

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1994 Apr 1
PMID 7517951
Citations 38
Authors
E Kokoskin
T W Gyorkos
A Camus
L Cedilotte
T Purtill
B Ward
Affiliations
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Abstract

Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.

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