The HNK-1 Epitope in the Inner Connective Tissue Layer of the Human Ciliary Body in Exfoliation Syndrome and Various Types of Glaucoma

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 1994 Jan 1

PMID 7509767

Citations 3

Authors

M Uusitalo

T Kivela

A Tarkkanen

Affiliations

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Abstract

Possible changes in the expression of the HNK-1 carbohydrate epitope in the inner connective tissue layer of the human ciliary body, located between the ciliary epithelium and muscle, was studied using 2 formalin-fixed, paraffin-embedded eyes with exfoliation syndrome, 33 eyes with different types of glaucoma, and 21 morphologically normal control eyes. A strong immunoreaction delineating cell process was observed in this layer with monoclonal antibodies HNK-1 and VC1.1 recognizing the HNK-1 epitope in control specimens, whereas partly granular immunoreaction was present in eyes with exfoliation syndrome. Exfoliation material was also immunoreactive. In all types of advanced glaucoma, the immunoreaction was mostly granular in nature and greatly diminished. No difference in HNK-1 immunoreactivity between control and glaucoma eyes was seen in the retina and ciliary epithelium. Elevated intraocular pressure, either directly or by decreasing blood flow to the ciliary body, may cause degenerative or metabolic changes in the inner connective tissue layer cells that bear or secrete molecules sharing the HNK-1 epitope. The partly granular immunoreactivity in eyes with exfoliation syndrome only indicates changes in this epitope even without an increase in intraocular pressure.

Citing Articles

The HNK-1 Carbohydrate Epitope and the Human Eye in Health and Disease.

Uusitalo M, Kivela T Pathol Oncol Res. 1997; 3(1):8-14.

PMID: 11173618 DOI: 10.1007/BF02893345.

Blood-aqueous barrier in pseudoexfoliation syndrome: evaluation by immunohistochemical staining of endogenous albumin.

Kuchle M, Vinores S, Mahlow J, Green W Graefes Arch Clin Exp Ophthalmol. 1996; 234(1):12-8.

PMID: 8750845 DOI: 10.1007/BF00186513.

Immunohistochemical localization of chondroitin sulfate proteoglycan and tenascin in the human eye compared with the HNK-1 epitope.

Uusitalo M Graefes Arch Clin Exp Ophthalmol. 1994; 232(11):657-65.

PMID: 7531167 DOI: 10.1007/BF00171380.

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