Structures of Cell Wall Mannans of Pathogenic Candida Tropicalis IFO 0199 and IFO 1647 Yeast Strains

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1994 Feb 1

PMID 7507898

Citations 12

Authors

H Kobayashi

K Matsuda

T Ikeda

M Suzuki

S Takahashi

A Suzuki

N Shibata

S Suzuki

Affiliations

Soon will be listed here.

Abstract

We conducted a structural analysis of the cell wall mannans isolated from two Candida tropicalis strains, IFO 0199 and IFO 1647, exhibiting strong agglutinabilities against anti-Candida factor sera 5 and 6. The products released from these mannans by acid treatment were identified as the oligosaccharides, from biose to pentaose, consisting solely of beta-1,2-linked mannopyranose units corresponding to common epitopes of Candida albicans serotypes A and B (factor 5). Mild acetolysis of acid- and alkali-treated mannans produced large amounts of hexaose and heptaose, Man rho beta 1-2Man rho beta 1-2Man rho alpha 1-2Man rho alpha 1-2Man rho alpha 1-2Man and Man rho beta 1-2Man rho beta 1-2Man rho beta 1-2Man rho alpha 1-2 Man rho alpha 1-2Man, corresponding to the C. albicans serotype A-specific epitopes (factor 6). However, the homologous pentaose, Man rho beta 1-2Man rho alpha 1-2 Man, was not generated by this procedure. The oligosaccharides (biose to hexaose) obtained from the mannans by conventional acetolysis were composed exclusively of alpha-1,2-linked mannopyranose units. Therefore, the mannans of C. tropicalis IFO 0199 and IFO 1647 do not have the alpha-1,3-linked mannopyranose units previously observed in the mannans of C. albicans and Candida stellatoidea. The results of this study and previous findings indicate that the similarity of the antigenicities of three Candida species, C. albicans serotype A, C. stellatoidea type II, and C. tropicalis, reside in the beta-1,2 and alpha-1,2 linkages containing oligomannosyl side chain (factor 6) in the cell wall mannan.

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References

Fukazawa Y, Shinoda T, Tsuchiya T . Response and specificity of antibodies for Candida albicans. J Bacteriol. 1968; 95(3):754-63. PMC: 252089. DOI: 10.1128/jb.95.3.754-763.1968. View

Ames B, DUBIN D . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960; 235:769-75. View

Shinoda T, Kaufman L, Padhye A . Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species. J Clin Microbiol. 1981; 13(3):513-8. PMC: 273824. DOI: 10.1128/jcm.13.3.513-518.1981. View

Ekwall E, Svenson S, Lindberg A . Identification of salmonellae of serogroup C1 by immunofluorescence and co-agglutination with antiserum against an oligosaccharide-protein conjugate. J Med Microbiol. 1982; 15(2):173-80. DOI: 10.1099/00222615-15-2-173. View

Shibata N, Ichikawa T, Tojo M, Takahashi M, Ito N, Okubo Y . Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide. Arch Biochem Biophys. 1985; 243(2):338-48. DOI: 10.1016/0003-9861(85)90511-9. View

Kobayashi H, Shibata N, Suzuki S . Acetolysis of Pichia pastoris IFO 0948 strain mannan containing alpha-1,2 and beta-1,2 linkages using acetolysis medium of low sulfuric acid concentration. Arch Biochem Biophys. 1986; 245(2):494-503. DOI: 10.1016/0003-9861(86)90242-0. View

Miyakawa Y, Kagaya K, Fukazawa Y, Soe G . Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. J Clin Microbiol. 1986; 23(5):881-6. PMC: 268742. DOI: 10.1128/jcm.23.5.881-886.1986. View

Reiss E, de Repentigny L, Kuykendall R, Carter A, Galindo R, Auger P . Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence. J Clin Microbiol. 1986; 24(5):796-802. PMC: 269031. DOI: 10.1128/jcm.24.5.796-802.1986. View

Lindberg B, Leontein K, Lindquist U, Svenson S, Wrangsell G, Dell A . Structural studies of the O-antigen polysaccharide of Salmonella thompson, serogroup C1 (6,7). Carbohydr Res. 1988; 174:313-22. DOI: 10.1016/0008-6215(88)85100-0. View

10.

Kwon-Chung K, Wickes B, Merz W . Association of electrophoretic karyotype of Candida stellatoidea with virulence for mice. Infect Immun. 1988; 56(7):1814-9. PMC: 259482. DOI: 10.1128/iai.56.7.1814-1819.1988. View

11.

AKSOYCAN N, Erdem B, Saganak I . The antigenic relationship between Candida (Torulopsis) glabrata and Escherichia coli (0:75, 0:163), Salmonella aberdeen O antigens. APMIS. 1988; 96(12):1143-4. DOI: 10.1111/j.1699-0463.1988.tb00993.x. View

12.

Kwon-Chung K, RIGGSBY W, Uphoff R, Hicks J, Whelan W, Reiss E . Genetic differences between type I and type II Candida stellatoidea. Infect Immun. 1989; 57(2):527-32. PMC: 313128. DOI: 10.1128/iai.57.2.527-532.1989. View

13.

Hamajima K, Nishikawa A, Shinoda T, Fukazawa Y . Detection of specificity of a new antigen in Candida tropicalis and its evaluation by taxonomic DNA analyses. Microbiol Immunol. 1988; 32(10):1013-24. DOI: 10.1111/j.1348-0421.1988.tb01466.x. View

14.

Kobayashi H, Shibata N, Mitobe H, Ohkubo Y, Suzuki S . Structural study of phosphomannan of yeast-form cells of Candida albicans J-1012 strain with special reference to application of mild acetolysis. Arch Biochem Biophys. 1989; 272(2):364-75. DOI: 10.1016/0003-9861(89)90230-0. View

15.

Kobayashi H, Shibata N, Nakada M, Chaki S, Mizugami K, Ohkubo Y . Structural study of cell wall phosphomannan of Candida albicans NIH B-792 (serotype B) strain, with special reference to 1H and 13C NMR analyses of acid-labile oligomannosyl residues. Arch Biochem Biophys. 1990; 278(1):195-204. DOI: 10.1016/0003-9861(90)90248-w. View

16.

Nnalue N, Weintraub A, Lindberg A . Properties of a rat monoclonal antibody reactive with both the mannan of Candida species and the O-antigen 6,7 polysaccharide of serogroup C1 salmonellae. Infect Immun. 1991; 59(1):229-33. PMC: 257731. DOI: 10.1128/iai.59.1.229-233.1991. View

17.

Kobayashi H, Giummelly P, Takahashi S, Ishida M, Sato J, Takaku M . Candida albicans serotype A strains grow in yeast extract-added Sabouraud liquid medium at pH 2.0, elaborating mannans without beta-1,2 linkage and phosphate group. Biochem Biophys Res Commun. 1991; 175(3):1003-9. DOI: 10.1016/0006-291x(91)91664-x. View

18.

Fukazawa Y . Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser. 1989; 47:37-62. View

19.

Shibata N, Kobayashi H, Takahashi S, Okawa Y, Hisamichi K, Suzuki S . Structural study on a phosphorylated mannotetraose obtained from the phosphomannan of Candida albicans NIH B-792 strain by acetolysis. Arch Biochem Biophys. 1991; 290(2):535-42. DOI: 10.1016/0003-9861(91)90578-7. View

20.

Kobayashi H, Kojimahara T, Takahashi K, Takikawa M, Takahashi S, Shibata N . Structural determination of D-mannans of pathogenic yeasts Candida stellatoidea type I strains: TIMM 0310 and ATCC 11006 compared to IFO 1397. Carbohydr Res. 1991; 214(1):131-45. DOI: 10.1016/s0008-6215(00)90536-6. View