Negative Regulation of Early B Lymphopoiesis by Interleukin 3 and Interleukin 1 Alpha

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1994 Jan 18

PMID 7507246

Citations 7

Authors

F Hirayama

S C Clark

M Ogawa

Affiliations

Soon will be listed here.

Abstract

We recently developed a two-step methyl cellulose culture system for murine lymphohemopoietic progenitors that are capable of differentiation along the myeloid and B-lymphoid lineages. In this system, two-factor combinations, which include steel factor plus interleukin (IL) 6, IL-11, or granulocyte colony-stimulating factor effectively supported the lymphomyeloid potential of primary colonies. Interestingly, IL-3 could neither replace nor act synergistically with steel factor in maintaining the B-lymphoid potential of the primary colonies although the frequency of colony formation was the same with IL-3 and steel factor. We now report that addition of IL-3 or IL-1 alpha to a permissive system suppresses the B-lymphoid potential of primitive progenitor cells in primary culture in dose-dependent fashion. In vivo transfer of the primary colonies to scid mice confirmed the suppressive effects of IL-3 and IL-1 alpha. In addition, IL-1 alpha inhibited pre-B-cell colony formation in the secondary culture. Once pre-B-cell colonies had formed in secondary culture, neither factor affected the proliferation of the pre-B cells. These results suggest negative regulatory roles for IL-3 and IL-1 alpha in early stages of B lymphopoiesis.

Citing Articles

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Inflammatory Changes in Bone Marrow Microenvironment Associated with Declining B Lymphopoiesis.

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Bone marrow fat and the decline of B lymphopoiesis in rabbits.

Kennedy D, Witte P, Knight K Dev Comp Immunol. 2015; 58:30-9.

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Inhibition of B Lymphopoiesis by Adipocytes and IL-1-Producing Myeloid-Derived Suppressor Cells.

Kennedy D, Knight K J Immunol. 2015; 195(6):2666-74.

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Negative cross-talk between interleukin-3 and interleukin-11 is mediated by suppressor of cytokine signalling-3 (SOCS-3).

Magrangeas F, Boisteau O, Denis S, Jacques Y, Minvielle S Biochem J. 2001; 353(Pt 2):223-30.

PMID: 11139384 PMC: 1221562. DOI: 10.1042/0264-6021:3530223.

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