The Structural Events Associated with the Attachment of Complement Components to Cell Membranes in Reactive Lysis

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1978 Aug 1

PMID 750370

Citations 18

Authors

R R Dourmashkin

Affiliations

Soon will be listed here.

Abstract

Electron microscopic study of the events occurring at the cell membrane during reactive lysis by complement, showed that a foliaceous particle was formed at the C5b-7 stage, that enlarged to a particle with a variable number of arms at the C5b-8 stage. Up to this point, no typical complement lesions were found. At the C5b-9 stages, the particles were completely converted to typical complement lesions, i.e. hollow cylinders projecting from the cell membrane and partly penetrating it. C5b-9 complexes assembled in the fluid phase did not show the typical structure of the lesions, but were amorphous masses of fibres.

Citing Articles

Two different complement Factor B (Bf) alleles of the orangutan major histocompatibility complex (MHC) are also conserved in chimpanzee and humans showing importance in primate immunity.

Arnaiz-Villena A, Juarez I, Vaquero-Yuste C, Lledo T, Martin-Villa J, Suarez-Trujillo F Mol Biol Rep. 2024; 52(1):6.

PMID: 39570459 DOI: 10.1007/s11033-024-10086-7.

Packing a punch: the mechanism of pore formation by cholesterol dependent cytolysins and membrane attack complex/perforin-like proteins.

Dunstone M, Tweten R Curr Opin Struct Biol. 2012; 22(3):342-9.

PMID: 22658510 PMC: 3383384. DOI: 10.1016/j.sbi.2012.04.008.

Structure of complement C6 suggests a mechanism for initiation and unidirectional, sequential assembly of membrane attack complex (MAC).

Aleshin A, Schraufstatter I, Stec B, Bankston L, Liddington R, Discipio R J Biol Chem. 2012; 287(13):10210-10222.

PMID: 22267737 PMC: 3323040. DOI: 10.1074/jbc.M111.327809.

Re-incorporation of the terminal C5b-9 complement complex into lipid bilayers: formation and stability of reconstituted liposomes.

Bhakdi S, Tranum-Jensen J Immunology. 1980; 41(3):737-42.

PMID: 7461711 PMC: 1458153.

Membrane attack complex of complement: a structural analysis of its assembly.

Podack E, Esser A, Biesecker G, MULLER-EBERHARD H J Exp Med. 1980; 151(2):301-13.

PMID: 7356725 PMC: 2185789. DOI: 10.1084/jem.151.2.301.

References

Borsos T, Dourmashkin R, Humphrey J . LESIONS IN ERYTHROCYTE MEMBRANES CAUSED BY IMMUNE HAEMOLYSIS. Nature. 1964; 202:251-2. DOI: 10.1038/202251a0. View

Shin M, Paznekas W, Abramovitz A, Mayer M . On the mechanism of membrane damage by C: exposure of hydrophobic sites on activated C proteins. J Immunol. 1977; 119(4):1358-64. View

HAMMER C, Shin M, Abramovitz A, Mayer M . On the mechanism of cell membrane damage by complement: evidence on insertion of polypeptide chains from C8 and C9 into the lipid bilayer of erythrocytes. J Immunol. 1977; 119(1):1-8. View

Packman C, Rosenfeld S, WEED R, Leddy J . Complement-induced ultrastructural membrane lesions: requirement for terminal components. J Immunol. 1976; 117(5 Pt.2):1883-9. View

KOLB W, MULLER-EBERHARD H . The membrane attack mechanism of complement. Verification of a stable C5-9 complex in free solution. J Exp Med. 1973; 138(2):438-51. PMC: 2139397. DOI: 10.1084/jem.138.2.438. View

Humphrey J . The nature of complement-induced lesions in membranes. Haematologia (Budap). 1972; 6(3):319-25. View

Sirchia G, Dacie J . Immune lysis of AET-treated normal red cells (PNH-like cells). Nature. 1967; 215(5102):747-8. DOI: 10.1038/215747a0. View

LACHMANN P, Thompson R . Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9. J Exp Med. 1970; 131(4):643-57. PMC: 2138770. DOI: 10.1084/jem.131.4.643. View

Thompson R, LACHMANN P . Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7. J Exp Med. 1970; 131(4):629-41. PMC: 2138783. DOI: 10.1084/jem.131.4.629. View

10.

LACHMANN P, BOWYER D, Nicol P, Dawson R, Munn E . Studies on the terminal stages of complement lysis. Immunology. 1973; 24(1):135-45. PMC: 1422877. View

11.

KOLB W, MULLER-EBERHARD H . The membrane attack mechanism of complement. Isolation and subunit composition of the C5b-9 complex. J Exp Med. 1975; 141(4):724-35. PMC: 2189744. View

12.

KOLB W, Haxby J, Arroyave C, MULLER-EBERHARD H . The membrane attack mechanism of complement. Reversible interactions among the five native components in free solution. J Exp Med. 1973; 138(2):428-37. PMC: 2139403. DOI: 10.1084/jem.138.2.428. View

13.

Humphrey J, Dourmashkin R . The lesions in cell membranes caused by complement. Adv Immunol. 1969; 11:75-115. DOI: 10.1016/s0065-2776(08)60478-2. View

14.

Valentine R, SHAPIRO B, Stadtman E . Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry. 1968; 7(6):2143-52. DOI: 10.1021/bi00846a017. View

15.

HAMMER C, Nicholson A, Mayer M . On the mechanism of cytolysis by complement: evidence on insertion of C5b and C7 subunits of the C5b,6,7 complex into phospholipid bilayers of erythrocyte membranes. Proc Natl Acad Sci U S A. 1975; 72(12):5076-80. PMC: 388878. DOI: 10.1073/pnas.72.12.5076. View

16.

Polley M, MULLER-EBERHARD H, FELDMAN J . Production of ultrastructural membrane lesions by the fifth component of complement. J Exp Med. 1971; 133(1):53-62. PMC: 2138882. DOI: 10.1084/jem.133.1.53. View

17.

LACHMANN P, Munn E, Weissmanng . Complement-mediated lysis of liposomes produced by the reactive lysis procedure. Immunology. 1970; 19(6):983-6. PMC: 1455672. View

18.

Frank M, Dourmashkin R, Humphrey J . Observations on the mechanism of immune hemolysis: importance of immunoglobulin class and source of complement on the extent of damage. J Immunol. 1970; 104(6):1502-10. View

19.

Rosse W, DOURMASHKIN R, Humphrey J . Immune lysis of normal human and paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. 3. The membrane defects caused by complement lysis. J Exp Med. 1966; 123(6):969-84. PMC: 2138177. DOI: 10.1084/jem.123.6.969. View